摘要
目的探讨神经节苷脂对缺血再灌注大鼠脑组织的保护作用。方法建立大鼠大脑中动脉栓塞再灌注模型,采用磁共振弥散加权成像(DWI)与磁共振波谱(MRS)技术,分别对假手术组、缺血再灌注组、神经节苷脂组大鼠的脑梗死体积、氮-乙酰天门冬氨酸(NAA)及乳酸(Lac)等代谢产物进行比较。结果神经节苷脂组在缺血再灌注1h、3h、6h时梗死区体积分别为(30.32±8.18)mm3、(35.17±12.45)mm3、(31.4±8.56)mm3,缺血再灌注组分别为(204.6±37.77)mm3、(218.9±67.33)mm3、(213.4±99.95)mm3,两组各时间点比较差异有统计学意义(均P<0.01)。神经节苷脂组在缺血再灌注1h、3h、6hLac/磷酸肌酸和肌酸(PCr+Cr)比值分别为0.09±0.11、0.15±0.18、0.13±0.22,缺血再灌注组分别为1.09±0.34、0.99±0.37、1.16±0.27,两组各时间点比较差异有统计学意义(均P<0.01);神经节苷脂组在缺血再灌注1h、3h、6hNAA/(PCr+Cr)比值分别为1.04±0.38、0.81±0.21、0.77±0.25,缺血再灌注组分别为0.55±0.23、0.57±0.12、0.58±0.13,各时间点两组比较差异有统计学意义(均P<0.01)。结论神经节苷脂通过抑制Lac的生成来间接减少Lac所导致的神经元细胞毒性脑水肿,从而显示其具有显著的脑保护作用。
Objective To observe the neuroprotective effect of Gangliosides on rats with cerebral ischemiareperfusion. Methods Rat models were established through middle cerebral artery occlusion and divided into shamoperation group, ischemia-reperfusion group and Gangliosides group. The volume of infarotion and changes of NAA and Lactate at different time point were evaluated by DWI and MRS examinations. Results The mean infarcted volume measured by DWI at 1 h, 3 h and 6 h after ischemia-reperfusion in Gangliosides group was （30.32 ±8.18） mm^3, （35.17 ± 12.45） mm^3 and （ 31.4 ± 8.56 ） mm^3, respectively, versus （ 204.6 ± 37.77 ） mm^3, （ 218.9 ± 67.33 ） mm^3 and （ 213.4 ± 99. 95 ） mm^3 in ischemia-reperfusion group. There were significant differences between the two groups （all P 〈0. 01 ）. The mean peak area ratio of lactate to creatine measured by MRS 1 h, 3 h, 6 h after ischemia-reperfusion was 0. 09 ± 0. 11, 0. 15 ± 0. 18, 0. 13 ± 0.22 in Gangliosides group, respectively, versus 1.09 ±0.34, 0.99±0.37, 1.16 ±0.27 in ischemia-reperfusion group （all P〈0.01）. The mean peak area ratio of NAA to Creatine measured by MRS 1 h, 3 h, 6 h after ischemia-reperfusion was 1.04 s0.38, 0.81 s0.21,0.77 ± 0.25 in Gangliosides group, respectively, versus 0.55 ± 0.23, 0.57 ± 0. 12, 0.58 ± 0. 13 in ischemia-reperfusion group （ all P 〈 0. 01 ）. Conclusions By inhibiting the formation of lactate and reducing indirectly brain neurons cytotoxic edema caused by lactate after cerebral ischemia-reperfusion, Gangliosides shows remarkable neuroprotective effect.
出处
《临床神经病学杂志》
CAS
北大核心
2008年第1期52-54,共3页
Journal of Clinical Neurology
关键词
缺血再灌注
神经节苷脂
磁共振弥散加权成像
磁共振波谱
ischemia-reperfusion
Gangliosides
diffusion-weighted magnetic resonance imaging
magnetic resonance spectroscopy
