摘要
目的:建立以高效液相色谱法同时测定蒙药草乌花中乌头碱、新乌头碱、次乌头碱含量的方法。方法:Extend-C18(250mm×4.6mm,5μm),流动相为甲醇-0.3mol/L三乙胺(65∶35),流速为0.8mL/min,检测波长为235nm,柱温为35℃。结果:乌头碱、新乌头碱、次乌头碱分别在1～5μg,0.35～1.75μg,0.5～2.5μg范围内呈良好的线性关系。结论:本方法简便、准确、分离效果好、线性范围宽、灵敏度高,可用于本品的质量控制。
Objective： To establish an HPLC method for simultaneous content determination of aconitine, mesaconitine, and hypaeonitine in Flos Aconiti kusnezoffii. Methods： Extend -C18 （250mm ×4. 6mm ,5 μm） was used as chromatographic column, methanol - 0.3 mol/L triethylamine （ 65 ： 35 ） as mobile phase. The detection wavelength was set at 235 nm, the flow rate at 0. 8mL/min and the column temperature at 35℃. Results ：The sample sizes of aconitine, mesaconitine, hypaconitine had good linear relationship with their peak areas in the range of 1 - 5μg, 0.35 - 1.75μg, and 0.5 - 2.5 μg, respectively. Conclusion ：The established method is convenient and accurate with satisfactory separation effect,wide linear range, and high sensitivity ,and can be available for the quality control of Aconitum kusnezoffii Reichb.
出处
《中华中医药学刊》
CAS
2008年第3期520-521,共2页
Chinese Archives of Traditional Chinese Medicine
基金
国家教育部科学技术研究重点项目(00167)
作者简介
图雅，女，2005级博士研究生。
通讯作者：张贵君，教授，博士研究生导师，研究方向：中药鉴定方法学，组分中药及中药药效组分质量标准研究。E—mail：guijunzhang@163．com。
