摘要
目的研究小干扰RNA(small interferingRNA,siRNA)抑制细胞周期蛋白D1(cyclin D1)对喉鳞状细胞癌(简称喉癌)细胞生长和凋亡的影响。方法通过脂质体转染试剂将针对cyclin D1的特异小干扰RNA转入喉癌细胞,利用MTT法检测喉癌细胞生长抑制情况,流式细胞技术检测喉癌细胞周期变化和细胞凋亡。结果MTT结果表明喉癌细胞生长受到抑制,并具有时间依赖性。同时实验组喉癌细胞周期受到影响,并检测到喉癌细胞的凋亡。结论Cyclin D1基因沉默后,喉癌细胞生长受到了抑制,细胞周期受到影响,并能够最终导致喉癌细胞凋亡。
OBJECTIVE To study the influence of small interfering RNA（siRNA）silencing cyclin D1 gene on cell proliferation and apoptosis in Hep-2 cell line. METHODS The special siRNA was transfected into Hep-2 cell line using liposomal transfection reagent. The inhibitory effects on the growth of Hep-2 cells were assessed by the MTT assay. Cell cycle analysis and quantization of apoptosis were valued by flow cytometry. RESULTS Silencing cyclin D1 could significantly suppress the growth of the Hep-2 cell in a time-dependent manner. The cell cycle arrest and apoptosis was detected. CONCLUSION Transfecting the special siRNA of cyclin D1 could effectively inhibit the growth of the canner cells, influence the cell cycle and induce their aooptosis.
出处
《中国耳鼻咽喉头颈外科》
北大核心
2008年第2期75-77,共3页
Chinese Archives of Otolaryngology-Head and Neck Surgery
基金
黑龙江省自然科学基金资助项目(D200656)
作者简介
通讯：肖玉丽，女，黑龙江人，医学硕士，主任医师，主要从事耳鼻咽喉头颈外科疾病的诊断和治疗。Email：gyq33200@yahoo．com
