摘要
目的:研制骨唾液酸蛋白-β-磷酸三钙(BSP-β-TCP)人工骨并观察BSP-β-TCP中骨唾液酸蛋白的释放规律。方法:实验于2006-12/2007-07在解放军广州军区广州总医院医学实验科完成。①BSP-β-TCP人工骨制备:采用低温负压冻干的方法将骨唾液酸蛋白复合于β-磷酸三钙中,按骨唾液酸蛋白含量不同制备两种样品:A样品含骨唾液酸蛋白1﹪,B样品含骨唾液酸蛋白2﹪。②观察指标:两种样品分别在恒温空气浴振荡器内(37±1)℃持续振荡。试验开始2周内每24h,以后每72h时分别吸出浸泡液100μL/次,共观察4周。用考马斯亮蓝法测定各个时间点溶液中蛋白质含量;绘制出蛋白质释放量和时间关系的散点图,再将散点图的曲线直线化,分析BSP-β-TCP人工骨中骨唾液酸蛋白的释放动力学。结果:①骨唾液酸蛋白释放率:72h内有一个爆发性的释放,释放率A样品为25.44﹪,B样品为35.11﹪;10d内的释放速度较快,A样品为39.76﹪,B样品为44.13﹪;持续4周仍有缓慢释放,A样品为55.43﹪,B样品为56.82﹪。②骨唾液酸蛋白的释放动力学:将散点图曲线进行拟和后可见释放量与时间(h)平方根成线性关系,两者的关系符合Higuchi方程。结论:骨唾液酸蛋白以扩散方式释放,β-磷酸三钙可作为良好的载体与BSP共同形成缓释系统。
AIM： To prepare bone sialoprotein- β -tricalcium phosphate （BSP- β -TCP） artificial bone and observe releasing rule of BSP in the artificial bone. METHODS： The experiment was carried out in the Medical Laboratory of Guangzhou General Hospital of Guangzhou Military Area Command of Chinese PLA between December 2006 and July 2007.（1）BSP in combination with β -TCP was conducted by using low temperature, negative pressure and freeze-dried device, to prepare BSP- β -TCP artificial bone. Two kinds of BSP- β -TCP artificial bone were produced according to the content of BSP： A sample （containing 1% BSP） and B sample （containing 2% BSP）. （2）The two samples were persis0. tently oscillated in constant temperatm-e air bath （37± 1）℃. The soak was inhausted 100μ L per time every 24 hours within two weeks after experiment, and then every 72 hours later, the observation lasted 4 weeks. Coomassie brilliant blue method was used to determine the protein content in solution at every time point. The scatterplot of relation between protein releasing amount and time was drawn, then lined curve of scatterplot was conducted to analyze releasing kinetics of BSP in BSP- β -TCP artificial bone. RESULTS： （1）BSP had an breaking out delivery in 72 hours, and the BSP release rate of A sample was 25.44% while that of B sample was 35.11%; BSP kept a high releasing rate in 10 days, 39.76% in A sample and 44.13% in B sample; slow releasing still remained for 4 weeks, the BSP release rate of A sample was 55.43%, and that of B sample was 56.82%.（2）After making the curve fitting of scatterplot, the linear correlation relationship between the release amount of BSP and the square root of time （hour） was found, in agreement with Higuchi equation. CONCLUSION： BSP release from BSP- β -TCP artificial bone in a diffuse manner. As a carrier, β -TCP can form slow-release system with BSP.
出处
《中国组织工程研究与临床康复》
CAS
CSCD
北大核心
2008年第1期27-30,共4页
Journal of Clinical Rehabilitative Tissue Engineering Research
基金
2005广东省科技三项经费计划项目(2005B34001008)~~
作者简介
王玮，男，1982年生，江西省德兴市人，汉族，2007年广州医学院毕业，硕士，医师，主要从事骨组织工程研究。myemai1306@tom.com.
通讯作者：章莹，博士，副主任医师，解放军广州军区广州总医院骨科，广东省广州市510010zhangying—doc@yahoo．com.cn
