人胚胎干细胞培养与诱导分化的研究进展及其存在问题

Advances of Study on Culture and Inductive Differentiation of Human Embryonic Stem Cells and Its Problems——Review

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摘要人胚胎干细胞(human embryonic stem cells,hESCs)指来源于人胚胎的具有无限自我增殖能力和多向分化潜能的细胞,主要由人囊胚内细胞群(innercellmass,ICM)分离得到。由于胚胎干细胞具有高度自我复制能力及多向分化潜能,可被应用于生命科学的多个领域。本文就人胚胎干细胞培养条件、体外诱导分化技术作一综述。 Human embryonic stem cells （hESCs） are cells with unlimited self-renewing property and differentiation potential mainly derived from the inner cell mass（ICM） of human blastocyst. Because of the remarkable developmental potential and proliferative capacity, human embryonic stem cells hold great promise for future use in various research areas, such as human developmental biology and cell-based therapy. This review expounds the culture system of human embryonic stem cells and the induced differentiation technology in vitro.

作者王磊顾健

机构地区徐州医学院扬州市血液学研究所

出处《中国实验血液学杂志》 CAS CSCD 2008年第1期227-230,共4页 Journal of Experimental Hematology

关键词人胚胎干细胞培养条件诱导分化 human embryonic stem cell culture system induced differentiation

分类号 R329.28 [医药卫生—人体解剖和组织胚胎学] Q253 [生物学—细胞生物学]

作者简介通讯作者：顾健，教授、主任医师、硕士生导师．电话：（0514）87937517．E-mail：maolujir018@yahoo．com．cn

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1Shamblott MJ, Axelan J, Wang S, et al. Derivation of pluripotent stem cells from cultured human primordial germ cells. Proc Natl Acad Sci USA, 1996; 95:13726-13731.
2Thomson JA, Itskovitz-Eldor J, Shapiro SS, et al. Embryonic stem cell lines derived from human blastocysts. Science, 1998; 282 (5391) :1145-1147.
3Amit M , Carpenter MK, lnokuma MS, et al. Clonally derived human embryonic stem cell lines maintain pluripotency and proliferative potential for prolonged periods of culture. Dev Biol, 2000; 227:271-278.
4Park JH, Kim SJ, Oh EJ, et al. Establishment and maintenance of human embryonic stem cells on STO, a permanently growing cell line. Biol Reprod, 2003; 69:2007-2014.
5Amit M, Margulets V, Segev H, et al. Human feeder Myers for human embryonic stem cells. Biol Reprod, 2003 ; 68:2150-2156.
6Hovatta O, Mikkola M, Gertow K, et al. A culture system using human foreskin fibroblasts as feeder cells allows production of hurman embryonic stem cells. Hum Reprod,2003 ; 18:1404-1409.
7Lee JB, Song JM, Lee JE, et al. Available human feeder cells for the maintenance of human embryonic stem cells. Reproduction, 2004; 128:727-735.
8Cheng L, Hammond H, Ye Z, et al. Human adult marrow cells support prolonged expansion of human embryonic stem cells in culture. Stem Cells,2003 ; 21 : 131-142.
9Amit M, Shariki C, Margulets V, et al. Feeder layer-and serumfree culture of human embryonic stem cells. Biol Reprod, 2004; 70:837-845.
10Xu C,Inokuma MS,Denham J,et al. Feeder-flee growth of undifferentiated human embryonic stem cells. Nat Biotechnol,2001 ; 19 :971-974.

1于佳.胚胎干细胞在动物科学中的应用[J].养殖技术顾问,2009(9):140-140.
2胡娅莉,徐运,崔恒宓,王心如.胚胎干细胞的研究进展[J].医学研究生学报,2005,18(1):77-80. 被引量：6
3王艳唯,王敬祥,岳福杰.胚胎干细胞在生物技术中的研究进展[J].畜牧兽医科技信息,2006,22(4):19-21.
4Wu CF Tsung HC Zhang W J Wang Y Lu JH Tang ZY Kuang YP Jin W Cui L Liu W Cao YL.Improved cryopreservation of human embryonic stem cells with trehalose[J].中国生物学文摘,2006,20(7):24-24. 被引量：2
5Wang Q Fang ZF Jin F Lu Y Gai H Sheng HZ.Derivation and growing human embryonic stem cells on feeders derived from themselves[J].中国生物学文摘,2006,20(7):29-29. 被引量：1
6Xiong C Xie CQ Zhang L Zhang J Xu K Fu M Thompson WE Yang L J Chen YE.Derivation of adipocytes from human embryonic stem cells[J].中国生物学文摘,2006,20(7):25-25.
7Huiming Xu,Weicheng Wang,Chunliang Li,Hongyao Yu,Acong Yang,Beibei Wang,Ying Jin.WWP2 promotes degradation of transcription factor OCT4 in human embryonic stem cells[J].Cell Research,2009,19(6):796-796. 被引量：2
8王士珍.胚胎干细胞的蛋白质组学研究[J].科技视界,2013(19):8-8.
9焦淑洁,许慧芳,许杰,湛彦强,张苏明.Effect of VEGF on Neural Differentiation of Human Embryonic Stem Cells in vitro[J].Journal of Huazhong University of Science and Technology(Medical Sciences),2009,29(5):563-566. 被引量：3
10Jizhong Zou,Rory Cochran,Linzhao Cheng.Double knockouts in human embryonic stem cells[J].Cell Research,2010,20(3):250-252.

中国实验血液学杂志

2008年第1期

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人胚胎干细胞培养与诱导分化的研究进展及其存在问题

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