摘要
采用甲醇法提取西兰花中硫代葡萄糖苷,利用重量法测定其中硫代葡萄糖苷的含量,得出硫代葡萄糖苷最佳提取条件,并对硫代葡萄糖苷提取液进行酸水解,通过抑菌圈实验,分析了西兰花中硫代葡萄糖苷水解产物异硫氰酸酯类的抑菌性。其结果表明,采用80%甲醇溶液、料液比为10:40(w/v)、80℃为提取的最佳条件,西兰花花蕾中硫代葡萄糖苷的含量最高,达到39.90μ mol/g;花茎中为18.28μ mol/g;叶中为11.86μ mol/g。实验结果表明异硫氰酸酯类对金黄色葡萄球菌(Staphylococcus aureus)、白葡萄球菌(White staphylococcus)、枯草芽孢杆菌(Bacillus subtilis)大肠杆菌(Escherichia coli)的抑菌作用均较明显。
Glucosinolates were extracted by methanol and determined by the weighting method from broccoli. The best conditions of Glucosinolates extract were obtained. And the Glucosinolates extract were acid-hydrolyzed, the antimicrobial character of hydrolysis products was analyzed though antimicrobial zone experiment. It was shown that the best conditions of the extract were 80%methanol, the feed ratio of 10：40（g/mL） and 80%. The highest content of the Glucosinolates was 39.90μmol/g in the buds of the broccoli, the second was 18.28μmol/g in the stem, the last was 11.86μmol/g in the leaves. The experiment result indicates that the isothiocyanates antimicrobial action to Staphylococcus aureus, White staphylococcus, Bacillus subtilis and Escherichia coli was obvious.
出处
《中国农学通报》
CSCD
2008年第2期335-338,共4页
Chinese Agricultural Science Bulletin
基金
大庆科技攻关项目"西兰花芽菜系列功能食品的开发"(SGG2006-014)
作者简介
钱丽丽，女，1979年出生，黑龙江大庆人，硕士，主要从事生物活性物质的提取与检测方向的研究。通信地址：163319黑龙江省大庆市八一农垦大学食品学院，E-mail：qianlili286@163．com。
通讯作者：裴世春，男，1966年出生，吉林人，博士，副教授，主要从事食品安全与检测方向的研究。通信地址：163319黑龙江省八一农垦大学食品学院食品科学系。E-mail：peisc2002cn@yahoo．com．cn。
