摘要
目的:探讨非甾体类抗炎药阿司匹林对存在有β-连环素异常表达的T淋巴细胞白血病Jurkat细胞株的作用及机制。方法:MTT法观察阿司匹林对Jurkat细胞的增殖抑制作用,流式细胞仪(FCM)检测阿司匹林对Jurkat细胞细胞周期分布的影响。实时荧光定量RT-PCR法分析经不同浓度的阿司匹林处理后的Jurkat细胞β-连环素及CyclinD1基因的表达水平的改变。结果:阿司匹林呈剂量依赖性抑制Jurkat细胞的增殖,72h半数抑制浓度为1.78mmol/L;随浓度增加,CyclinD1基因及蛋白的表达水平均下调。结论:非甾体类抗炎药阿司匹林可能通过影响Wnt信号通路调节某些细胞周期相关基因的表达,将Jurkat细胞阻滞于G0/G1期,从而抑制白血病细胞株Jurkat的增殖。
Objective：To explore the effects of aspirin on proliferation of T-acute lymphoblastic leukemia cells lines Jurkat in vitro. Method：Jurkat cells were treated with aspirin at different concentrations in culture. Growth inhibition was detected by MTT assay, The changed cell cycles distribution of Jurkat cells induced by aspirin was analysed by flow cytometry. The expression of β-catenin and CyclinD1 were detected by SYBR Green Ⅰ -based real -time quantitative RT-PCR. Result： Aspirin treatment caused a dose-dependent inhibition of J urkat cell proliferation, with a 72 h IC50 of 1.78 mM. Aspirin could commit Jurkat cells to G0/G1 phase arrest. At the same time, aspirin treatment induced a decrease in the expression level of CyclinD1 mRNA and expression level of CyclinD1 protein. Conclusion： Non-steroidal anti-inflammatory drugs aspirin treatment could inhibit proliferation of Jurkat cells by decreasing abnormal expression of CyclinD1.
出处
《临床血液学杂志》
CAS
2008年第1期35-38,共4页
Journal of Clinical Hematology
