摘要
目的探讨不同浓度L-精氨酸(L-Arg)加入心脏停搏液对婴幼儿心肌缺血/再灌注的影响。方法择期行室间隔缺损(VSD)修补术患儿48例,随机分为对照组(C组,n=16)、L-ArgI组(L1组,n=16)和L-ArgⅡ组(L2组,n=16)。L1组停搏液中加入2.5g/L的L-Arg,L2组停搏液中加入5g/L的L-Arg,C组停搏液中加入相同容积的生理盐水。分别于诱导后(基础值,T0)、主动脉开放5min(T1)、主动脉开放30min(T2)、停体外循环(CPB)4h(T3)、停CPB24h(T4)抽取动脉血,测血浆心肌肌钙蛋白I(cTnI)、丙二醛(MDA)、超氧化物歧化酶(SOD)水平。结果3组再灌注后血浆cTnI、MDA浓度均显著升高(P<0.01),而SOD活性显著降低(P<0.01或P<0.05)。L1组cTnI、MDA升高幅度低于C组,SOD降低幅度低于C组(P<0.05或P<0.01)。L2组与C组比较无明显差异(P>0.05)。结论含L-Arg2.5g/L的心脏停搏液对婴幼儿心肌缺血/再灌注损伤有保护作用,含L-Arg5g/L的心脏停搏液对这种损伤无明显影响。
Objective To investigate the effects of different densities of L-arginine cardioplegic solution on infant myocardial ischemia-reperfusion injury. Methods Forty eight patients scheduled for repair of ventricular septal defect （VSD） were randomly divided into three groups： L-Arg Ⅰ group（L1 group, n = 16）received L-Arg 2.5 g/L in cardioplegic solution, L-ArgII group（L2 group, n = 16）received L-Arg 5g/L in cardioplegic solution and control group （group C, n = 16）received saline solution in thesame mode. Blood samples in artery were taken after induction（T0 ）,5 min after opening aorta（T1 ）, 30min after opening aorta（T2 ）, 4 h after termination of CPB （T3） ,24 h after termination of CPB （T4） for determination ofthe levels of cTnI, MDA and SOD. Results The levels of cTnI and MDA of three groups increased aftermyocardial ischemia-reperfusion, but the levels of cTnI and MDA were significantly lower in L1 group than those in control group. The activity of SOD of three groups decreased after myocardial ischemia-reperfusion, but the activityof SOD in L1 group was significantly higher than that of control group. There was no significant difference in the activity of SOD between L1 group and control group. Conelusian 2.5 g/L L-Arg cardioplegic solution has protective effect on infant myocardial ischemia-reperfusion injury, however, 5 g/L L-Arg cardioplegic solution has no effect on infant myocardial ischemia-reperfusion injury.
出处
《河北医药》
CAS
2008年第1期29-31,共3页
Hebei Medical Journal
基金
河北省科学技术研究与发展指导计划(编号:062761181)
