摘要
目的研究缺氧复合NaCN中毒对大鼠心功能的影响。方法雄性SD大鼠84只,随机均分为平原实验组和高原实验组。平原实验组大鼠在常规实验室内进行实验,高原实验组大鼠置4000m低压舱中预处理3d后开始实验。两组各取6只大鼠麻醉后进行气管和左侧颈总动脉插管,术后静待30min后,背部皮下注射NaCN3.6mg/kg,记录中毒前10min(-10min)、中毒即刻及中毒后10、20、30、40、50、60、90min各时相点左心室收缩压峰值(mLVSP)、左心室压力上升最大速率(+dp/dtmax)和心率(HR);余下两组各36只大鼠皮下注射3.6mg/kgNaCN,于中毒即刻及中毒后0.5、1、2、4、6h取动脉血测定心肌酶谱(AST、LDH、CK、CK-MB)。结果与平原实验组比较,高原实验组大鼠NaCN中毒后mLVSP、+dp/dtmax、HR、心肌酶谱的变化更为明显且更难以恢复正常。心肌酶谱中以LDH变化最为显著,这与缺氧环境下无氧呼吸相应增强一致。结论高原缺氧后NaCN中毒对大鼠心脏功能影响较平原更为严重,需要进一步探索相应的防治措施。
Objective To approach the effects of combined plateau hypoxia due to high altitude and sodium cyanide （NaCN） intoxication on the heart function of rats. Methods 84 healthy male SD rats were randomly divided into 2 groups： only NaCN intoxication in plain areas （altitude 308m） （PL group） and combined high altitude （altitude 4 000m） hypoxia and NaCN intoxication （PH group）. Rats in PH group were pre-treated with hypoxia for 72h in a plateau hypoxia cabin （simulating altitude 4 000m）. 12 rats of PH group were anesthetized. NaCN in a dosage of 3. 6mg/kg was subcutaneously injected into the back of rats. At the time points of -10min, 0min, 10min, 20min, 30min, 40min, 50rain, 60min and 90rain after NaCN injection, the left ventricular systolic pressure （mLVSP）, ＋dP/dtmax and heart rate （HR） were recorded by physiological functions signal processing system RM6240. The arterial blood were taken from the other 72 rats for myocardium zymograrn detection （including AST, LDH, CK, CK-MB） at the time points of Oh, 0. 5h, lh, 2h, 4h and 6h after NaCN injection. Results Compared with the PL group, the mLVSP, ＋dP/dt and HR changed obviously （P〈0. 01） in the rats in PH group with the influences of plateau environment, and these changes were difficult to recover, meanwhile the myocardium zyrnograrn of rats in PH group showed significant changes accordingly. The most distinguish changes were on LDH that was in accordance with the lack of oxygen in hypoxia circumgtance. ~mdusion The condition of combined hypoxia and NaCN intoxication would bring about more severe consequence to body, and impair the cardiac function acutely. Search for effective measures for prevention and therapy is necessary for the treatment.
出处
《解放军医学杂志》
CAS
CSCD
北大核心
2008年第2期126-128,共3页
Medical Journal of Chinese People's Liberation Army
基金
全军医药卫生“十一五”科研基金资助项目(06G077)
关键词
缺氧
氰化钠
心脏功能试验
anoxia
sodium cyanide
heart function tests
作者简介
唐禾，博士生。主要从事防化医学的基础研究
【通讯作者】董兆君，E-mail：dongzj@163．com
