摘要
目的观察低蛋白饮食(lowproteindiet,LPD)以及LPD+α-酮酸(α-ketoacid,α-KA)对腺嘌呤致慢性肾功能衰竭(chronicrenalfailure,CRF)大鼠模型营养状况及肾间质纤维化的作用。方法雄性Wistar大鼠46只分对照组(n=10),CRF模型组(n=36,以腺嘌呤150mg/kg.d灌胃6w制模)。CRF模型组造模成功后随机分正常蛋白(normalproteindiet,NPD)组、LPD组和LPD+α-KA组,于10w、14w末测定血肌酐(Scr)与尿素氮(BUN)、血浆钙(Ca)、磷(P)、白蛋白(Alb)、血红蛋白(Hb)、24h尿蛋白(24hUPro);Masson染色分析肾间质纤维化程度;免疫组化方法测定肾组织中α平滑肌肌动蛋白(α–SMA),RT-PCR方法测定肾组织α–SMAmRNA表达程度。结果CRF各组体重增长、Alb、Hb、Scr水平均无差异。10w时,LPD及LPD+α-KA组BUN水平显著低于NPD组。10w时,CRF各组Ca、P水平无差异。14w时,LPD及LPD+α-KA组血Ca显著高于NPD组,CRF各组血P无差异。10w、14w时,LPD及LPD+α-KA组24hUPro明显低于NPD组。CRF各组间质纤维化程度逐渐加重,LPD、LPD+α-KA组显著轻于NPD组;10w时,LPD与LPD+α-KA间差异不显著;14w时,LPD+α-KA组较LPD组轻(P<0.05)。10w时,LPD与LPD+α-KA比NPD组肾组织α-SMA表达减轻,两者之间没有差别,14w这种作用不明显。结论LPD+α-KA及LPD均能维持CRF动物营养状态正常,均能减缓肾间质纤维化,LPD+α-KA的作用更强。这可能与肾小管上皮-间充质转化(epithelialmesenchymaltransition,EMT)程度减轻有关。
Objective To evaluate the effects of low proteins diet （LPD） supplemented with ketoacids（α-KA） on the nutritional status and renal interstitial fibrosis in comparison with conventional LPD in adenine induced chronic renal failure （CRF） rat model. Method Forty-six male Wistar rats were divided into two parts： normal control rats （n=10） and CRF rats（n=36）, which were treated with 150 mg/（kg bw · d） adenine intragastrically. After 6 w, CRF rats were divided into three groups： （a） normal protein diet NPD （20% casein diet） group, n=12; （b） LPD （6% casein diet） group, n=12; （c） LPD＋α-KA （5%casein .＋1% α-KA）group, n=12. All the rats were sacrificed at 10,14 w. Body weight（BW）, hemoglobin（Hb）, albumin （Alb）, serum creatinine（Scr）, blood urea nitrogen （BUN） , 24h urine protein（24hUpro） were measured.Masson stain was performed to grade the extent of renal interstitial fibrosis. Immunohistochemistry and RT-PCR methods were used to evaluate the extent of expressions of α- smooth muscle actin （α-SMA）. Results The levels of Scr, Hb and BW were not different among the three groups over time. 24hUpro in both LPD and LPD＋α-KA groups were lower significantly than in NPD group. BUN levels of LPD and LPD＋α-KA group were significantly lower than that in NPD group at 10w. Serum Ca levels of LPD and LPD＋α-KA groups were significantly higher than that in NPD group at 14w. The extent of renal fibrosis in LPD and LPD＋α-KA groups was milder than that in NPD group, much milder in LPD＋α-KA （P〈0.05）. Expressions of a-SMA in LPD and LPD＋α-KA group were also lower than that in NPD group. Conclusion LPD and LPD＋α-KA not only sustain the nutritional status, but also ameliorate the renal fibrosis and epithelial-mesenehymal transition （EMT）in adenine-induced CRF rat model, and these effects of LPD＋α-KA are better than LPD. [ACTA NUTRIMENTA SINICA, 2008,30（1）： 61-65]
出处
《营养学报》
CAS
CSCD
北大核心
2008年第1期61-65,共5页
Acta Nutrimenta Sinica
关键词
慢性肾衰
低蛋白
α-酮酸饮食
肾间质纤维化
Α-平滑肌肌动蛋白
chronic renal failure
low protein diet （LPD）
, α-Ketoacid （α-KA）
renal interstitial fibrosis
a-smooth muscle actin（α-SMA）
作者简介
刘燕萍（1970-），女，主治医师，硕士，E-mail：liuypl227@vip．Sina．com；
通讯作者：郑法雷，E-mail：zhengf146@vip．sina．com
