摘要
目的：用RP-HPLC法测定三七片中三七皂苷R1和人参皂苷Rg1的含量。方法：以ZORBAXEXTEND-C18分析柱（4.6mm×250mm,5μm）;乙腈-水（24∶76）为流动相;检测波长203nm;流速1.2mL.min^-1;柱温30℃。结果：三七皂苷R1在0.153-0.918μg内与峰面积呈良好的线性关系（r=0.99805）;人参皂苷Rg1在0.786-4.716μg内与峰面积呈良好的线性关系（r=0.99949）;回收率三七皂苷R1为97.15%,RSD=0.93%,人参皂苷Rg1为98.25%,RSD=0.68%。结论：方法简便、快速、重现性好,可作为三七片质量控制的辅助检测选项。
Objective ：Determine the notogiensenoside R1 and giensenoside Rgl in Sanqi Tablets by RP - HPLC. Methods ： ZORBAX EXTEND - C18 column （4.6mm × 250mm,5μm） was used as a stationary phase , aeetonitrile - water （ 24：76 ） as a mobile phase , and detection wavelength at 203nm, 1. 2mL·min^-1 of flow rate and 30℃ column temperature. Results： The calibration curves were linear in the range of 0. 153 - 0.918μg for notoginsenoside R1 （ r = 0.99805） and 0.786 - 4. 716 μg for ginsenoside Rgl （ r = 0.99949 ） . The Recovery of notoginsenoside R1 was 97.15% with RSD 0.93% , and that of ginsenoside Rgl was 98.25% with RSD 0.68%. Conclusion： The method is simple and quick with good reproducibility . It can be used as adjuvant determination for the quality control of Sanqi Tablets.
出处
《中华中医药学刊》
CAS
2008年第2期421-422,共2页
Chinese Archives of Traditional Chinese Medicine
作者简介
俞坚（1970-），女，浙江杭州人，主管中药师，学士，研究方向：医院药学、中药鉴别。
