摘要
副溶血性弧菌(Vibrio parahaemolyticus)是一种重要的食源性致病菌,广泛存在于各种海产品中。由于传统培养基和检测方法费时费力,本研究设计开发了一种新型显色培养基HKC vibrio,通过应用于人工污染样品和实际样品检验,以法国科玛嘉弧菌显色平板CHROMagar vibrio和柠檬酸钠-硫代硫酸钠-氯化钠-蔗糖琼脂平板(TCBS)为对照,对显色培养基HKC vibrio的灵敏性、特异性和检测效果进行了初步评价。结果表明,HKC vibrio的灵敏性与CHROMagar vibrio和TCBS相当,并具有较好的特异性,HKC vibrio是非常有价值的分离平板,可大大提高副溶血性弧菌的检测效率。
Vibrio parahaemolyticus is an important food-borne pathogenic bacterium that widely exists in all kinds of seafood. As the traditional media and method were very costly-time and money, a new chromogenic medium （HKC vibrio） was designed in this assay. Through detecting the artificially contaminated samples and natural samples, the sensitivity, specificity and detection effect of HKC vibrio were studied and compared with CHROMagar vibrio and TCBS. The results showed that HKC vibrio had the same sensitivity as CHROMagar vibrio and TCBS, also had highly specificity. In conclusion, HKC vibrio was an invaluable medium which may improve the detection efficiency of Vibrio parahaemolyticus dramatictly.
出处
《微生物学通报》
CAS
CSCD
北大核心
2008年第1期145-148,共4页
Microbiology China
基金
广州市农业科技招标项目(No.GZCQC0602FC3001C)
关键词
副溶血性弧菌
显色培养基
检测效果
初步评价
Vibrio parahaemolyticus, Chromogenic medium, Detection effect, Primary evaluation
作者简介
通讯作者：Tel：020—87688I32；E-mail：wuqp@gdas．ac．cn
