摘要
目的建立术芍颗粒的质量标准。方法采用TLC法对处方中的甘草和平地木进行定性鉴别;应用HPLC对方中芍药苷进行含量测定,制定合理的含量下限。结果本品定性鉴别薄层色谱特征明显,专属性强;本品中芍药苷在0.316～1.580μg内线性关系良好(r=0.9999),平均回收率为98.64%,RSD=1.24%。本品中芍药苷含量下限为0.43%。结论薄层色谱鉴别和含量测定方法准确可行,重复性好,可有效地控制术芍颗粒的质量。
OBJECTIVE To establish the quality standard for Zhushao granules. METHODS Radix Glycyrrhizae and Herba Ardisiae Japonicae were identified by TLC; HPLC was used to determine paeoniflofin content, and the reasonable lower limit of the content was established. RESULTS The chromatogram for identification by TLC was distinct and hightly specific. A good linear relationship was obtained in a range of paeoniflorin concentrations from 0.316 μg to 1. 580 μg （r = 0.999 9）, The average recovery was 98.64%, RSD was 1.24% ; The lower limit of the content of paeoniflorin was 0.43%. CONCLUSION The methods of TLC identification and quantification were accurate, realizable and reproducible and can be used effectively for the quality control of Zhushao granules.
出处
《中国现代应用药学》
CAS
CSCD
北大核心
2007年第6期489-491,共3页
Chinese Journal of Modern Applied Pharmacy
作者简介
王冰，女，硕士，助研 Tel：（021）58956227 E-mail：annabel-cn@sina．com
