摘要
目的:探讨经肝动脉使用干扰素α1b-碘化油乳剂对家兔移植VX2肝癌血管内皮生长因子(VEGF)及肿瘤生长的影响。方法:通过开腹接种法在日本大白兔左前叶肝脏建立荷瘤兔模型,2周后经彩色多普勒超声检查肝脏有肿瘤生长无腹水且血供丰富的兔作为研究对象。Ⅰ组(n=10):仅行经股动脉肝动脉插管;Ⅱ组(n=10):行经肝动脉超液态碘油栓塞;Ⅲ组(n=10):行经肝动脉干扰素α1b-碘化油乳剂栓塞。观察家兔的麻醉苏醒至正常进食时间及其一般情况。14天后处死家兔,测量原发肿瘤的大小、计数肝内转移灶的数目、腹腔有无转移、免疫组织化学染色检测VEGF的表达。结果:家兔麻醉苏醒至正常进食时间Ⅱ、Ⅲ组间无明显差异(P>0.05),但较Ⅰ组明显延长(P<0.05);其它一般情况无明显差异;肝内平均原发灶大小Ⅱ、Ⅲ组间无明显差异(P>0.05),较Ⅰ组明显缩小(P<0.05);肝内平均转移灶数目Ⅱ组比Ⅲ组明显增多(P<0.05),Ⅰ、Ⅲ组间及Ⅰ、Ⅱ组间无明显差异(P>0.05);肿瘤内及正常肝组织内测VEGFⅠ、Ⅲ两组之间无明显差异(P>0.05),Ⅱ组VEGF的阳性率明显高于第Ⅰ、Ⅲ组(P<0.05)。结论:经肝动脉栓塞肝癌可能促进VEGF的表达而导致肝内转移,干扰素α1b-碘化油乳剂行肝动脉栓塞可抑制单纯栓塞治疗后VEGF的增强表达,控制原发灶生长和肝内转移。
Objective： To investigate effect of vascular endothelial growth factor（VEGF） and tumor growth after transarterial chemoembolization with interferon α1b-lipiodol emulsion in a rabbit model bearing VX2 liver tumor. Methods： To establish tumor model in the left anterior lobe of the liver of the Japanese white rabbits via laparotomic route. Thirty rabbits were successfully implanted and were hypervascular detected by color Doppler ultrasound after 2 weeks. Then were divided into 3 groups randomly, 10 in each group. In group Ⅰ, only angiography was performed after percutaneous trans-femoral retrograde approach of hepatic arterial catheterization. In group Ⅱ, transcatheter arterial embolization with 0.6ml super liquid iodinated oil were carried out after hepatic angiography. In group Ⅲ, 0.6ml super liquid iodinated oil and interferon α1b 7.5×10^6U emulsion were given to each rabbit instead of super liquid iodinated oil. To observe the period from resuscitation of anesthesia to normal eating and common condition after surgery. All the rabbits were killed 14 days postoperatively. The tumor sizes were measured, the numbers of metastasis in liver were calculated. Condition of metastasis in abdominal cavity was observed. The expression of VEGF in the specimen that was obtained in tumor liver tissue and surrounding normal liver tissue（4 groups perrabbit） was examined by immunohistochemistry. Results： Differences of the mean period from resuscitation of anesthesia to normal eating among the group Ⅱ and Ⅲ were not significant （P〉0.05）, but significantly more prolongation than group Ⅰ （P〈0.05）. Other common condition was not significant. Differences of the sizes of primary tumor among the group Ⅱ and Ⅲ were not significant （P〉0.05）, but significantly smaller than group Ⅰ （P〈0.05）. The number of metastasis in group Ⅱ was more than that of group Ⅰ and Ⅲ（P〈0.05）; in group Ⅰ and Ⅲ the group Ⅰ and Ⅱ the number of metastasis were not signifcant （P〉0.05）. The expression of VEGF in the tumor and normal liver tissue were not significant in group Ⅰ and Ⅲ （P〉0.05）. In group Ⅱ the expression of VEGF was significantly higher than that in group Ⅰ and Ⅲ（P〈0.05）. Conclusion： Transcatheter arterial chemoembolization （TAE） probably promotes the expression of VEGF and metastasis in liver. TAE with interferon α1b-lipiodol emulsion could inhibit the expression of VEGF after merely TAE, inhibit tumorous growth and prevent metastasis in liver.
出处
《中国临床医学影像杂志》
CAS
北大核心
2008年第1期30-34,共5页
Journal of China Clinic Medical Imaging
关键词
肝肿瘤
实验性
兔
血管内皮生长因子A
干扰素Α
栓塞
治疗性
Liver neoplasms, experimental
Rabbit
Vascular endothelial growth factor A
Interferon-alph
Embolization, therapeutic
作者简介
斯光晏（1972-），男，四川西充人，主治医师。
[通讯作者]周石，贵阳医学院附属医院放射科，550004。
