摘要
建立了适合水稻花粉离体萌发的液体培养基配方,并用花粉萌发和碘.碘化钾(I_2-KI)染色测定法检测了喷施苯达松后含敏感致死基因的水稻恢复系‘Mc526’的花粉生活力。结果表明,苯达松处理前期(施后1～4d),‘Mc526’花粉活力与未作处理的接近;处理后期(施后5～8d)略低于未作处理的,但差异不显著。苯达松对母本结实率影响不显著。
A solution medium for pollen germination of rice （Oryza sativa） in vitro was established. After bentazon treatment, the pollen viability of rice restorer line ＇Mc526＇, which included bentazon sensitive lethal （bsl） gene, was researched by using pollen germination and I2-KI pigmentation. The results showed that in the early treated period （1-4 d）, the pollen viability of ＇Mc526＇ was similar to the control. And in the later period （5- 8 d）, the pollen viability was a bit lower than the control, but the difference was not obvious. It indicated that bentazon had no salient influence on the setting rate of sterile line.
出处
《植物生理学通讯》
CAS
CSCD
北大核心
2007年第6期1072-1076,共5页
Plant Physiology Communications
基金
安徽省科技攻关(重大)项目(06003010B)
安徽省教育厅自然科学研究项目(2005KJ169)
作者简介
通讯作者（E-mail：yjianbo@263．net：Tel：0551-2160212）。
