摘要
目的探讨肿瘤坏死因子相关凋亡诱导配体(TRAIL)联合奥沙利铂(oxaliplatin)对结肠癌细胞株SW480凋亡的影响,并初步探讨其作用机制。方法应用MTT法分别检测TRAIL组、奥沙利铂组及联合组的细胞抑制率,AnnexinⅤ-FITC/PI染色,流式细胞仪检测SW480细胞的凋亡率。结果SW480细胞对TRAIL不敏感,对奥沙利铂相对敏感,TRAIL与亚毒性浓度的奥沙利铂联用对细胞的杀伤作用显著增强;亚毒性浓度的TRAIL、奥沙利铂及联合组作用SW480细胞24h的抑制率分别为8.62%,60.51%,81.28%;凋亡率分别为2.43%,11.76%,18.37%,流式细胞学证实这种杀伤作用主要通过诱导细胞凋亡实现。结论TRAIL能显著提高奥沙利铂对结肠癌细胞的杀伤作用,此协同作用主要是通过诱导肿瘤细胞凋亡来实现。
Objective To determine the effect and mechanism of tumor necrosis factor - related apeptosis - inducing ligand （TRAIL） combined with oxaliplatln on apoptosis of human colorectal cancer cell line SW480. Methods Proliferation inhibitory rate of TRAIL and oxaliplatin was examined by methyl thiazolyl tetrazolium （MTT） assay. AnnexinV - FITC and Propidium iodide （PI） were used to stain SW480 cells, and flow cytometry （FCM） was used to determine apoptosis after treatment of TRAIL and/or oxaliplatin. Results SW480 cells were not sensitive to TRAIL but relatively sensitive to oxaliplatin with dose - dependent cytotoxicity. Combination therapy of TRAIL and subtoxic level of oxaliplatin resuited in a synergistic cytotoxic effect. The proliferation inhibitory rate after 24 - hour treatment of 100 ng/ml TRAIL, 100 μg/ml oxaliplatin and TRAIL plus oxaliplatin was 8. 62%, 60. 51% and 81.28% respectively while the apeptosis rate was 2. 43%, 11.76% and 18. 37%. The synergistic cytotoxicity was high partly attributed to cell apeptosis, which was proved by simultaneous flow cytometry assay. Conclusion TRAIL combined with subtoxic level of oxaliplatin enhances the killing effect in SW480 cell, which is mainly attributed to cell apoptosis.
出处
《广东医学》
CAS
CSCD
北大核心
2008年第1期34-36,共3页
Guangdong Medical Journal
基金
广东省自然科学基金资助项目(编号:06020005)
作者简介
通讯作者。E-mail：ezhongt@126．com
