摘要
2005年从山东某鹅场16日龄商品代病死鹅中分离到1株鹅细小病毒(Goose parvovirus,GPV),命名为SD2005株。用建立的荧光定量PCR(FQ-PCR)检测鹅细小病毒的方法,检测SD2005株人工感染3日龄雏鹅(GPV抗体阴性)后各器官不同时间段的病毒含量,结果表明:在攻毒8h后能从消化系统及血液、心脏和肝脏中检测到病毒DNA;病毒DNA的含量在48h后达到最高峰,并一直维持到168h。随后各待检脏器中病毒DNA含量持续降低,但在720h后仍能从粪便、肝脏和脾中检测到少量的病毒DNA。本试验为阐明GPV的致病机理及防控提供了重要的试验数据和理论依据。
Fluorescence quantitative PCR （FQ-PCR） assay was developed for rapid detection of Goose parvovirus. Three-day old goslings were artificially infected by Goose parvovirus （GPV） SD2005 strain that was locally isolated from a 16-day-old dead goose. The distribution of the virus in the gosling body was detected by FQ-PCR. The results were as follows： At hour 8 post-infection （PI）, the GPV DNA could be detected from digestive organs, blood, heart and liver. The GPV DNA reached peak quantity at 48 h PI, and could be detected from all organs. The fastigium persisted up to 168 h PI and then declined quickly. At 720 h PI, however, the GPV DNA could only be detected from feces, liver and spleen. This experiment provides a foundation for investigation of pathogenic mechanism of GPV infection.
出处
《中国预防兽医学报》
CAS
CSCD
北大核心
2008年第1期64-67,共4页
Chinese Journal of Preventive Veterinary Medicine
基金
国家人事部博士后基金
山东省自然基金项目(Y2006D09)
关键词
人工感染
鹅细小病毒
荧光定量PCR
体内分布
artificially-infect
goose parvovirus
fluorescence quantitative PCR
distribution
作者简介
毕建敏（1981-），女，山东威海人，硕士研究生，主要从事动物微生物与免疫学研究。
通讯作者：朱瑞良 E-mail：zhurl@sdau．edu．cn
