摘要
目的尝试制备一种咳嗽变异性哮喘(CVA)的豚鼠模型。方法30只雄性豚鼠饲养1周后,随机分为健康正常组和模型组,每组15只。模型组第1天肌注4%OVA溶液0.5ml,同时腹腔注射2%Al(OH)30.2ml,14d后,模型组用1%OVA溶液雾化后攻击,隔天1次,每次15s,共7次。第29天,两组豚鼠用10-4mol/L辣椒素溶液引咳。结果通过对咳嗽次数、肺病理切片、肺泡灌洗液、外周血等指标的观测,发现模型组的咳嗽次数多于健康正常组;与健康正常组比较,模型组的病理损伤明显;从肺泡灌洗液和血内嗜酸性粒细胞计数及白细胞计数上看,模型组与健康正常组两组存在统计学差异,前者较后者计数均有升高。结论这种改进后方法能成功地建立咳嗽变异性哮喘模型。
Objective To establish a cough variant asthma （CVA） model in guinea pig. Methods Thirty male guinea pigs were randomly divided into two groups： normal group（n= 15） and model group（n = 15）. The guinea pigs in model group were sensitized with intramuscular injection of 4% ovalbumin solution 0.5 ml and intraperitoneal injection of AI（OH）3 solution 0.2 ml at day 1. After 14 d, the guinea pigs were challenged with aerosolized 1% ovalbumin（OVA） solution for 15 s every other day . At day 29, all guinea pigs were challenged with aerosolized 10^-4 mol/L capsaicin solution for violent cough and corresponding pathological alteration . Results Compared with normal animals , theguineapigsinmodelgroup had higher cough frequency [ （ 7.82 ± 2.44 ） vs （3.67±3.16）,P=0.004], higher white blood cell（WBC） counts[（2.44±0.91） × 109/L vs （1.66±0.56） × 10^9/L, P=0.034] and eosinophils（EOS） counts[ （0.12 ± 0.07） × 109/L vs （0.05 ± 0.03） × 10^9/L, P = 0. 002 ] in bronchoalveolar lavage, higher WBC counts [（11.09±2.26）× 109/L vs （8.30± 1.90） × 109/L,P = 0.006] and IK）S counts[（0.12±0.06） × 10^9/L vs（0.08± 0.03） × 10^9/L, P = 0. 014] in blood. There was significant pathological impairment in model group. Conclusion CVA model can be established successfully by this method.
出处
《山西医科大学学报》
CAS
2007年第12期1070-1073,共4页
Journal of Shanxi Medical University
关键词
哮喘
豚鼠
模型
动物
asthma
guinea pig
model, animals
作者简介
蔡黎，女，1978-04生，硕士，住院医师．
