摘要
目的:探讨温通针法对血管性痴呆(VD)大鼠的疗效机制。方法:健康Wistar大鼠50只,随机分为正常组、模型组、药物组、温通针法组和捻转针法组。采用反复夹闭双侧颈总动脉-再灌注方法造成VD大鼠模型,并给予相应的针刺("大椎""合谷"等)及药物(尼莫地平)治疗15d,采用放射免疫学方法检测大鼠血浆中降钙素基因相关肽(CGRP)及内皮素(ET)含量的变化。结果:VD模型组大鼠CGRP含量较正常组显著降低(P<0.01),ET含量较正常组显著升高(P<0.01);温通针法组、捻转针法组和药物组分别与模型组比较,均能明显升高CGRP含量(P<0.05,0.01),降低ET含量(P<0.01);温通针法组与药物组相比差异无显著性意义(P>0.05),且温通针法组升高CGRP含量、降低ET含量的作用优于捻转针法组(P<0.01)。结论:温通针法能使血浆中ET含量降低、CGRP含量升高,因而温通针法能有效改变机体中舒缩血管物质的含量,从而改善脑组织血液供应。
Objective： To observe the effects of warming-promotion needling （WPN） on the levels of plasma calcitonin gene-related peptide （CGRP） and endothelin （ET） contents in vascular dementia （VD） rats so as to investigate its underlying mechanism in improving VD. Methods： A total of 50 Wistar rats were randomized into normal control, model, medication, WPN, and needle-twirling groups, with 10 cases in each. VD model was established by intraperitoneal injection of sodium nitro- prusside （2.5 mg/kg） in combination with repeated occlusion of the bilateral common carotid arteries and reperfusion. For rats in medication group, Nimodipine （0.0108 g/kg） was given （oral perfusion）, and for animals in WPN and needle-twirling groups “Dazhui” （GV 14）, “Baihui” （GV 20） and “Shuigou” （GV 26） were punctured manually by manipulating the needle with WPN and common twirling methods respectively, 60 s for each acupoint. The treatment was given once daily for 15 days. Plasma CGRP and ET contents were measured by radioimmunoassay. Results： Compared with normal control group, plasma CGRP content in model group decreased considerably （P〈0.01）, while plasma ET level in model group increased significantly （P〈0.01 ）. In comparison with model group, CGRP contents of WPN, needle-twirling and medication groups increased significantly （P〈0.05, 0.01 ）, while plasma ET levels of WPN, needle-twirling and medication groups decreased significantly （ P〈0.01 ）. The therapeutic effects of WPN group were significantly superior to those of needle-twirling group in raising CGRP content and lowering ET level （P〈0.01）; and the therapeutic effect of medication group was markedly superior to that of needle-twirling group in lowering ET content （P〈0.01）. No significant differences were found between WPN and medication groups in raising plasma CGRP and lowering ET level, and between needle-twirling and medication groups in raising CGRP content （P〉0.05）. Conclusion： Warming-promotion needling can effectively lower plasma ET and raise plasma CGRP level in VD rats, which may contribute to its clinical effect in improving VD patients＇ memory-learning ability.
出处
《针刺研究》
CAS
CSCD
2007年第6期380-383,共4页
Acupuncture Research
作者简介
杨晓波（1967-），女，硕士，副主任医师，讲师，主要从事传统针刺手法的临床与实验研究工作。E-mail：yangxiaobo538@sina.com
