摘要
目的以原核表达的MPB70-MPB83-CFP10-ESAT-6融合蛋白作为诊断抗原,建立牛结核病抗体检测间接ELISA(iELISA)。方法用iELISA检测90份健康牛血清,确定样本阴阳性临界值(S/P)为0.17。iELISA与商品化胶体金试剂条(ICG)平行检测150份临床奶牛血清样本;以结核菌素皮内试验(TST)为参考方法,检测了华中地区四个奶牛场的560头中国黑白花奶牛和90份进口奶牛结核阴性血清;对其中22头奶牛进行鼻拭子分菌检验,4头分菌阳性奶牛的血清抗体检测也为阳性。结果iELISA与ICG的符合率为93.33%(140/150),与TST的总符合率为87.32%(489/560),与细菌培养的总符合率为77.27%(17/22)。以TST为参考方法,iELISA的敏感性和特异性分别为72.37%和89.67%。结论该方法具有较高的灵敏度与特异性,与其它方法有较高的符合率。
The prokaryotically expressed tetra-valent fusion protein（MPB70-MPB83-CFP10-ESAT6）was used as the diagnostic antigen in the indirect ELISA assay to detect the specific antibodies occurring in bovine tuberculosis,in which the cut-off value（S/P）as determined with indirect ELISA in serum samples from 90 healthy cows was found to be 0.17,and the rate of agreement between the indirect ELISA and the commercial colloidal gold-strip test reached up to 9⒊33%.Taken the tuberculin skin test（TST）as reference,560 serum samples from 4 local cow herds in the center of China were assayed with indirect ELISA.It was found that the rate of agreement between indirect ELISA and TST was 87.32%（489/560）and that with isolation of bacteria in nasal swabs was 77.27%（17/22）.The sensitivity and specificity of this indirect ELISA assay were proved to be 72.37% and 89.67% respectively.It is evident this method of indirect ELISA has high sensitivity,specificity and rate of agreement with tuberculin skin test.
出处
《中国人兽共患病学报》
CAS
CSCD
北大核心
2007年第11期1123-1126,共4页
Chinese Journal of Zoonoses
基金
"973"计划重要传染病基础研究专项(2006CB504400)
国家"十一五"科技攻关奶业重大专项(项目编号:2006BAD04A05
2006BAD04A12)
武汉市科技攻关课题(20066002056)
作者简介
通讯作者：郭爱珍,Email：aizhen@mail．hzau．edu．cn
