摘要
以直径在200～300μm的腔前卵泡为研究对象，研究了不同分离方法及卵巢不同发育状况对猪腔前卵泡分离及体外培养的影响。结果表明，显微分离法每个卵巢可采集的卵泡数极显著高于剪碎过滤法（24．75±2．99vs2．60±0．35，P〈0．01），显微分离法分离每个腔前卵泡所用的时间极显著低于剪碎过滤法（5．40±0．44vs15．52±1．18，P〈0．01）；有黄体和无黄体的卵巢对卵泡分离的影响差异不显著（26．10±2．02vs24．35±2．32，P〈0．05），但有黄体的卵巢分离的卵泡数稍多于无黄体的卵巢。此外，对两种分离方法分离出的腔前卵泡进行培养研究表明，两种分离方法对卵泡体外生长和存活，以及正常卵母细胞比率均没有显著影响（P〉0．05）。
By using secondary follicle of diameter of 200 - 300μm as an object, we observed the effect of isolation methods and the development of ovary on the isolation and collection of porcine secondary follicles. The results indicated that the number of secondary follicle isolated by microdissection is highly significantly more than that of cutting filtration （24.75 ± 2.99 vs 2.60 ±0.35, P 〈 0.01 ）, the time every follicle costs by microdissection is highly significantly lower than that of cutting filtration（5.40 ±0.44 vs 15.52 ±1.18, P 〈 0.01 ）. There is no significance of collection of follicle between ovary with corpus luteum and without corpus luteum when both using microdissection method（26.10 ± 2.02 vs 24.35 ± 2.32, P 〉 0.05）, but ovary with corpus luteum will isolate little more follicles than ovary without corpus luteum. In addition, we examined the effect of different isolation method on the in vitro culture of follicles. The results showed that there is no significant difference on the growth, survival and normal oocytes （ P 〉 0.05）.
出处
《石河子大学学报（自然科学版）》
CAS
2007年第4期456-459,共4页
Journal of Shihezi University(Natural Science)
关键词
猪
腔前卵泡
分离
卵巢
体外培养
porcine
secondary follicle
isolation
ovary
in vitro culture
作者简介
权青（1980-），男，硕士生，研究方向为动物胚胎工程：e-mail：qqing336@sina．com。
通讯作者：章孝荣（1954-），男，教授，博士，从事动物胚胎工程和动物生殖内分泌研究：e-mail：zxr@ahau．edu．cn。
