摘要
采用微型的毛细管电泳-安培（CE-AD）测定装置对市售的3种无糖口香糖中的木糖醇和山梨醇的测定进行了研究。实验采用铜盘工作电极，在0．65V的检测电位下，用70mmol／LNaOH缓冲溶液，4kV的分离电压，木糖醇和山梨醇在10min之内实现了良好的分离，其中木糖醇的线性范围为5×10^-5～1×10^-2mol／L，山梨醇的线性范围为5×10^-5～5×10^-2mol／L（r〉0．9997），检出限分别为5×10^-6mol／L和2．5×10^-6mol／L。该方法测定样品中木糖醇和山梨醇的相对标准偏差和回收率分别为3．7％、4．5％和98．1％、91．1％（n=10）。将该方法用于好丽友口香糖、益达口香糖、华艾康口香糖中木糖醇和山梨醇的测定，结果令人满意。
A miniaturized capillary electrophoresis with electrochemical detection （CE-ECD） system was developed for the separation and determination of xylitol and sorbitol in three commercial sugar-free gums. Factors influencing the separation and detection processes were examined and optimized. These two analytes have been separated within 10 min at a separation voltage of 4 kV in 70 mmol/L NaOH running buffer. Linear response was obtained at the range of 5.0×10^-5 - 1×10^-2mol/L and 5.0×10^-5 - 5.0×10^-2 mol/L with the detection limits （S/N = 3 ） of 5.0 ~ 10-6mol/L and 2.5×10^-6 mol/L for xylitol and sorbitol, respectively （ r 〉 0. 9997）. The proposed method was successfully applied to determine the gum samples with the RSD and average recoveries of 3.7%, 4.5% and 98.1%, 91.1% for xylitol and sorbitol, respectively （n = 10）.
出处
《分析化学》
SCIE
EI
CAS
CSCD
北大核心
2007年第11期1661-1664,共4页
Chinese Journal of Analytical Chemistry
关键词
微型化
毛细管电泳-电化学
无糖口香糖
木糖醇
山梨醇
Miniaturization, capillary electrophoresis-amperometric detection, sugar-free chewing gums, xylitol, sorbitol
作者简介
E-mail：weihongz@email．jlu．edu．cn
