摘要
目的:提高复合针介导的大豆遗传转化效率。方法:以萌发1 d的半片大豆种子作为目标组织,用复合针对其子叶节组织进行刺伤处理,并接种于含有pCAMBIA1201载体的根癌农杆菌LBA4404中;采用GUS染色法测定瞬时表达效率。结果:刺伤处理中,用复合针刺伤2次时,转化效率最高,平均每个外植体达到9个蓝色斑点;基因型对转化效率也有较大影响,所试5个品种中,合丰25的转化效率最高,平均为7.3个蓝色斑点;共培养时间以5 d为宜;添加抗氧化剂1.0 mmol/L二硫苏糖醇+1.0 mmol/L硫代硫酸钠+3.3 mmol/L半胱氨酸时,能够明显提高瞬时转化效率。结论:提高了复合针介导的大豆遗传转化效率。
Objective： To enhance the efficiency of the multi-needle-assisted transformation of soybean（Glycine max L. Merr.）. Methods： The cotyledonary node cells of half seeds germinated for 1 d were wounded by a multi-needle and inoculated with Agrobacterium tumefaciens LBA4404 harboring a vector pCAMBIA1201. The transformation efficiency was determined by the GUS activity. Results： Among wounding treatments, the highest transformation efficiency was obtained when wounding was made by puncturing 2 times with the multi-needle. The transformation efficiency was also significantly affected by genotypes, co-cultivation time and antioxidants. Conclusion： The efficiency of the multi-needle-assisted soybean transformation was increased.
出处
《生物技术通讯》
CAS
2007年第6期946-948,共3页
Letters in Biotechnology
基金
山东省教育厅基金项目(J05K04)
作者简介
谢宏峰（1981-），男，硕士研究生
[通讯作者]薛仁镐，（E-mail）rengaox@163．com
