摘要
目的:建立大鼠原位肝移植(ROLT)急性排斥反应模型,观察细胞毒淋巴细胞抗原4免疫球蛋白(CTLA-4Ig)在大鼠肝移植术后对共刺激分子B7-1/B7-2的影响及其抗排斥反应的作用。方法:采用"二袖套管"法先行建立DA-Lewis大鼠组合肝移植急性排斥反应模型,随机分为对照组(A组)与实验组(B组)两组,于肝移植术后48h每只受体大鼠腹腔内一次性注射CTLA-4Ig 75μg,分别于术后3、5、7和10d采用RT-PCR检测B7-1和B7-2 mRNA在两组肝脏组织中的表达情况,并同时观察其肝功能变化。结果:1)B7-1和B7-2 mRNA在A组高水平表达,而在B组表达明显降低(P<0.01);2)B组动物术后未见明显排斥反应,血清ALT、TBIL和DBIL水平明显低于对照组(P<0.01)。结论:移植术后应用CTLA-4Ig可以降低肝组织中B7-1和B7-2的表达;动态检测B7分子的表达有助于观察肝移植排斥反应的进程。
Objective： To observe the effect of CTLA-4lg on preventing acute rejection and the role of costimulatory molecule B7-1 and B7-2 via the model of orthotopic liver transplantation （ROLT） in rats. Methods：Build the model of ROLT （DA- Lewis,）. All rats were divided into 2 groups randomly.Group A was control group. Group B was injected with CTLA4-lg into abdominal cavity after 48 hours of operation. Animals were sacrificed on the day 3,5,7,10 after ROLT to detect the changes of serum biochemistry （ALT, TBIL and DBIL）.Liver grafts were collected for measuring the expression of B7-1 and B7-2 mRNA by RT-PCR.Results：1）Compared with the control group, the expression of B7-1 and B7-2 mRNA increased significantly（P〈 0.01）. 2）In group B, no clinical rejection character were obserbed and serum biochemistry was lower than cortol group （P〈0.01）. Conclusion： CTLA-4lg treatment can abate the expression of B7-1 and B7-2 mRNA. The dynami determination of costimulatory molecule B7 is helpful to the observation of rejection course in rats.
出处
《中国现代普通外科进展》
CAS
2007年第5期382-385,共4页
Chinese Journal of Current Advances in General Surgery
基金
教育部博士点基金(20050422058)
山东省自然科学基金资助项目(Y2003c15)
作者简介
刘钊（1975．11-），山东济南人，硕士，济南市中心医院主治医师，研究方向：肝胆外科。Tel：13805311761 E—mail：Lzconquer@yahoo．com．cn
[通讯作者]徐克森，山东大学齐鲁医院普外科，教授。Tel：13969059666
