摘要
采用单因素试验研究培养时间、培养温度和固水比对米曲霉(Aspergillus oryzae)AO-01产糖化酶的影响。在此基础上,再采用Box-Behnken响应面法(response surface methodology,RSM)对以上培养条件进行了优化。通过对二次多项回归方程求解得知,在上述自变量分别为培养时间32.8h,培养温度31.2℃,固水比1:1.24时,糖化酶的产量从最低值656.2μ/g干曲提高到1645.1μ/g干曲。验证实验证实了方程的预测值与实际值之间具有较好的拟合度。
The effects of incubation time, temperature and moisture content on the production of glucoamylase by Aspergillus oryzae AO-01 were investigated on the results of one-factor at a time method. Then a response surface methodology （RSM） was used to optimize the above critical external factors, so as to find out the optimal conditions and the relationships among these factors. By solving the quadratic regression model equation with appropriate statistic methods, the optimal conditions of cultivation time, temperature and ratio of substrate to water are 32.8 h, 31.2 ℃ and 1：1.24 respectively. The production of the glucoamylase is increased from the lowest value of 656.2 U/g to 1645.1U/g wheat Qu. The experimental data under various conditions have validated the theoretical values.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2007年第11期322-327,共6页
Food Science
基金
长江学者和创新团队计划资助项目(IRT0532)
江苏省"青蓝工程"资助项目
绍兴市重点科研工业项目(2006A21036)
作者简介
李旺军（1979-），男，硕士研究生，主要从事传统酿造微生物资源的研究。
通讯作者
