摘要
目的:分析单人操作大鼠原位肝移植(rat orthotopic liver transplantation,ROLT)模型建立术的学习曲线。方法:将作者学习制作该模型最初的100例资料分为5阶段(各20例),对操作时间,无肝期,手术成功率,长期存活率及并发症率进行分析。结果:Ⅲ,Ⅳ,Ⅴ阶段操作无肝期较Ⅰ,Ⅱ阶段明显减少(P=0.000),手术成功率从35%稳定上升至100%,长期存活率经历Ⅲ阶段55%～60%的平台期,达到95%;并发症率大大降低,模型稳定。结论:初学者经过耐心反复的训练,可通过学习曲线,熟练掌握ROLT术,成功建立肝移植基础研究需要的各种模型。
Objective：To analyze the learning curve of establishing the model of othotoptic liver transplantation in rat by a single operator. Methods：The data of the first 100 consecutive cases of rat orthotopic liver transplantation（ROLT） model were collected for this retrospective study. The models were devided into five phages, of which each group were 20 cases. The donor and recipient operation time, anhepatic time,operation success rate,long-term survival rate and complications rate of each stage were observed and analyzed. Results：The anhepatic times of stage Ⅲ,Ⅳ and Ⅴ decreased significantly compared with the first two stages（P = 0.000）. The operation success rate elevated from 35% to 100% steadily and the long-term survival rate raised to 95% with a plat-form survival rate of 55%-60% included（stageⅢ）. The complications rate decreased significantly, and the ROLT model was established stably. Conclusion：Learners could grasp these skills by the learning curve with patient and training repeatedly, after which any model suitable for experimental study of liver transplantation could be established successfully.
出处
《南京医科大学学报（自然科学版）》
CAS
CSCD
北大核心
2007年第9期924-926,共3页
Journal of Nanjing Medical University(Natural Sciences)
基金
江苏省卫生厅"135"工程基金资助(135-54)
关键词
肝移植
模型
学习曲线
大鼠
liver transplantation
model
learning curve
rat
作者简介
李相成,通讯作者,E-mail：drlxc@njmu.edu.cn
