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激光诱导荧光微流控芯片光纤检测系统 被引量:2

An Optical Fiber Detection System for Micro-fluidic Chip Laser-Induced Fluorescence
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摘要 基于反射式激光诱导荧光技术,以半导体激光器(中心波长532nm)为激发光源,研制了微流控芯片激光诱导荧光光纤检测系统。采用光纤束传感器使检测系统结构简单、紧凑、抗干扰力强,采用光子计数方法提高检测灵敏度。以罗丹明B为检测物质,实验发现荧光峰值明显,重复检测性好,其最小检出限为5×10^-12mol/L,线性范围为10^-6~10^-12mol/L。 Based on reflecting laser-induced fluorescence,an optical fiber detecting system for micro-fluidic chip laser induced fluorescence using solid-state (532 nm) as excitation source is developed. The use of optical fiber detector makes the system simple,compact and high anti-disturbing. And the photon count method is used to improve the sensitivity of the detection. Using rhodamine B as the sample. The detection limit and the linear dynamica are 5×10^-12 mol/L and 10^-5-10^-12 mol/ L. distinct fluorescence signal with good repetition is showed in the experiment.
出处 《光电子.激光》 EI CAS CSCD 北大核心 2007年第11期1356-1358,共3页 Journal of Optoelectronics·Laser
基金 广东省自然科学基金资助项目(05300261) 广东省科技计划资助项目(2005B10201001)
关键词 激光诱导 荧光 光纤束传感器 微流控芯片 laser-induced fluoresence optic fiber beam sensor micro-fluidic chip
作者简介 王洪(1964-),男,博士,副教授,主要从事光通信网络与光纤感技术方面的研究.E-mail:phhwang@scut.edu.cn
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  • 1任吉存,邓锡云,曹亚,姚开泰.毛细管电泳-激光诱导荧光法分离检测DNA片段及基因扩增产物[J].高等学校化学学报,1996,17(3):362-366. 被引量:6
  • 2[27]Men Z J, Qi S Z, Soper S A, et al. Interfacing a Polymer-based Micromachined Device to a Nanoelectrospray Ionization Fourier Transform Ion Cyclotron Resonance Mass Spectrometer. Anal. Chem., 2001, 73: 1286~1291
  • 3[28]Yuan C H, Shiea J. Sequential Electrospray Analysis Using Sharp-tip Channels Fabricated on a Plastic Chip. Anal. Chem., 2001, 73: 1080~1083
  • 4[29]Johnson T J, Ross D, Gaitan M, et al. Laser Modification of Preformed Polymer Microchannels: Application to Reduce Band Broadening Around Turns Subject to Electrokinetic Flow. Anal. Chem., 2001, 73: 3656~3661
  • 5[31]Weinert A, Amirfeiz P, Bengtsson S, et al. Plasma Asisted Room Temperature Bonding for MST. Sensors and Actuators A, 2001, 92: 214~222
  • 6[32]McCreedy T. Fabrication Techniques and Materials Commonly Used for the Production of Microreactors and Micro Total Analytical Systems. Trends in Analytical Chemistry, 2000, 19(6): 396~401
  • 7[33]Nakanishi H, Nishimoto T, Nakamura R, et al. Studies on SiO2-SiO2 Bonding with Hydrofluoric Acid. Room Temperature and Low Stress Bonding Technique for MEMS. Sensors and Actuators A, 2000, 79: 237~244
  • 8[35]Weiller B H, Ceriotti L, Shibata T, et al. Analysis of Lipoproteins by Capillary Zone Electrophoresis in Microfluidic Devices: Assay Development and Surface Roughness Measurements. Anal. Chem., 2002, 74: 1702~1711
  • 9[36]Takoshi I, Kaiuharu S, Seishiro O, Water Glass Bonding for Micro-total Analysis System. Sensors and Actuators B, 2002, 81:187~195
  • 10[37]Chiem N, Harrison D J. Microchip-based Capillary Electrophoresis for Immunoassays: Analysis of Monoclonal Antibodies and Theophylline. Anal. Chem., 1997, 69: 373~378

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