摘要
目的观察不同剂型表没食子儿茶素没食子酸酯[(-)-epigallocatechin-3-gallate,EGCG]对慢性中波紫外线(UVB)辐射后BALB/C小鼠表皮细胞凋亡的影响。方法不同剂型EGCG(溶液、乳膏及脂质体)局部外用于小鼠背部皮肤后给予30mJ.cm-2的UVB照射,每天1次持续30d,TUNEL法检测小鼠表皮中的凋亡细胞。结果不同剂型的EGCG均表现为促凋亡作用,乳膏和脂质体组效果强于溶液组(P<0.05),乳膏组和脂质体组之间未显示统计学差异(P>0.05)。结论EGCG对慢性低剂量UVB辐射后的小鼠表皮细胞有促凋亡作用,乳膏和脂质体效果较溶液显著。
OBJECTIVE To investigate the influence of （ - ）-epigallocatechin-3-gallate （EGCG） in different formulation on the apoptosis of epidermal cells of BALB/C mice irradiated by chronic UVB. METHODS Dorsal skin of BLAB/C mice were treated with EGCG in different formulation （solution,cream and liposome） and then irradiated with UVB in the dosage of 30 mJ ·cm^-2 once a day for 30 d. The apoptotic cells were detected by TUNEL. RESULTS EGCG in all formulation promoted apoptosis. The effect of EGCG cream and liposome were better than that of solution （P 〈 0. 05 ）. There was no statistic significance between EGCG cream and liposome groups （P 〉 0. 05 ）. CONCLUSION EGCG promoted the apoptosis of epidermal cells of BALB/C mice after chronic UVB radiation at low dosage. The effect of EGCG cream and liposome were better than that of solution.
出处
《中国药学杂志》
CAS
CSCD
北大核心
2007年第20期1540-1542,共3页
Chinese Pharmaceutical Journal
基金
国家自然科学基金资助项目(30371292)
作者简介
徐晶，女，硕士，住院医师
通讯作者：骆丹，女，教授，博士生导师 E-mail：daniluo2005@yahoo.com.cn.
