摘要
目的:探讨上皮性卵巢癌组织中环氧化酶-2(cyclooxygenase-2,COX-2)基因表达及其临床意义。方法:用逆转录-聚合酶链法(RT-PCR)检测32例上皮性卵巢癌、10例良性卵巢肿瘤和10例正常卵巢组织中COX-2 mRNA的表达,分析其与临床病理参数、化疗耐药之间的关系,同时对预后进行多因素的Cox生存分析。结果:COX-2 mRNA在上皮性卵巢癌中的表达(68.8%)显著高于良性卵巢肿瘤(0%)及正常卵巢组织(0%)(P<0.05),COX-2 mRNA的表达与淋巴结转移、术后残留灶直径有关(P<0.05),与临床分期、组织学分级、有无腹水均无关(P>0.05)。COX-2 mRNA在耐药组和非耐药组中的表达差异有统计学意义(P<0.05)。多因素生存分析显示淋巴结转移、术后残留灶直径和COX-2表达是影响患者预后的独立危险因素。结论:COX-2基因在上皮性卵巢癌的发生、发展、转移和化疗耐药中发挥重要作用,COX-2的表达是影响患者预后的独立危险因素。
Objective：To investigate the expression of cyclooxygenase-2（COX-2） in epithelial ovarian carcinoma and its clinical significance. Methods：The expression of COX-2 mRNA was detected by RT-PCR in 32 cases of epithelial ovarian carcinoma, 10 cases of benign ovarian tumors and 10 cases of normal ovarian tissues. The correlation of COX-2 expression to the clinicopathological parameters and chemotherapy resistance were analyzed. Multivariate Cox analysis on prognostic factors was performed. Results：The expression of COX-2 mRNA （68.8%） in epithelial ovarian carcinoma was statistically higher than that in the benign ovarian tumors （0%） and the normal ovarian tissues（ 0%, P 〈 0.05 ）. There was a significant correlation between COX-2 mRNA expression and lymphatic metastasis and the diameter of residual tumor （ P 〈 0.05 ）. But COX-2 mRNA transcription had no association with the clinical stages, histological grades, ascites occurrence （ P 〉 0.05 ）. The difference was significant in COX-2 mRNA transcription between the drug sensitive group and the chemoresistant group（ P 〈 0.05 ）. According to Multivariate Cox survival analysis, COX-2 expression, lymphatic metastasis, and the diameter of residual tumor were independent risk factors for predicting the prognosis of epithelial ovarian carcinoma （P 〈 0.05）. Conclusion： COX-2 plays an important role in the oncogenesis, development, metastasis, and chemoresistance of epithelial ovarian carcinoma. The expression of COX-2 is an independent risk factor for predicting the prognosis of patients.
出处
《肿瘤》
CAS
CSCD
北大核心
2007年第10期825-828,共4页
Tumor
基金
湖北省科技攻关项目(编号:2005AA304B05)
关键词
卵巢肿瘤
环氧化酶2
基因表达
逆转录合酶链反应
预后
Ovarian neoplasms
Cyclooxygenase 2
Gene expression
Reverse Transcriptase polymerase chain reaction
Prognosis
作者简介
夏杨（1981-），女（汉族），硕士研究生
Correspondenceto：WU Xu—feng（吴绪峰）Tel：027-67812648 E—mail：xy9901259@163．com
