摘要
目的:观察hBMP-7基因转染对Beagle犬骨髓基质细胞(BMSC)生物学特性的影响。方法:利用脂质体介导法将hBMP-7基因转染Beagle犬BMSC,观察细胞形态及生长情况,检测碱性磷酸酶、骨钙素及钙化结节等成骨细胞表型。结果:目的基因转染后细胞形态略有变化,增殖能力无明显改变,凋亡率无明显变化,碱性磷酸酶活性明显增高,骨钙素表达增强,钙化结节增大。结论:hBMP-7基因转染可以加速BMSC向成骨细胞表型转化。hBMP-7基因转染的BMSC有望成为牙周组织工程理想的种子细胞。
AIM：To investigate the effects of human BMP -7 gene transfection on bone marrow stromal cells （BMSC） in vitro. METHODS： BMSC were transfected with hBMP -7 gene through lipofectamineTM 2000. Morpho- logical and growth feature of the transfected cells were evaluated. Alkaline phosphatase （ALP）, osteocalcin（OC） and Von Kossa were tested. RESULTS： Compared to the control group, morphological feature of the transfected cells changed a little, but there were no significant differences of cell proliferation and apoptosis rate. ALP activity and expression of OC were increased significantly, as well as the size of calcium nodules. CONCLUSION： The transfection of hBMP-7 gene promoted the differentiation of BMSC to osteoblasts. BMSC transfected by hBMP- 7 gene may be served as an ideal sourceof cell for periodontal tissue engineering.
出处
《牙体牙髓牙周病学杂志》
CAS
2007年第10期558-562,共5页
Chinese Journal of Conservative Dentistry
基金
国家自然科学基金资助项目(30471892)
福建省自然科学基金资助项目(C0410024)
福建医科大学科学研究发展基金资助项目(XZ04011)
关键词
骨形成蛋白-7
基因治疗
骨髓基质细胞
组织工程
bone morphogenetic protein- 7
gene therapy
bone marrow stromal cells
tissue engineering
作者简介
李艳芬（1979-），女，汉族，福建寿宁人。博士生（导师：同福华），住院医师。
通讯作者：闫福华，E—mail：fhyan2005@126．com．Tel：0591—83736426
