摘要
建立了同时检测化妆品中24种防腐剂含量的反相高效液相色谱法（RP—HPLC）。采用KromasilC18（4．6mm×250mm，5μm）色谱柱，以磷酸盐缓冲溶液（pH=4．26）为流动相，梯度洗脱。样品经甲醇超声提取，然后采用RP—HPLC-二极管阵列检测法测定，对样品前处理和色谱条件进行研究和优化。方法的相对标准偏差为1．3％～3．3％；回收率为90．6％～97．8％；各种防腐剂的线性相关系数≥0．9997，具有较高的准确度和精密度，可用于化妆品中24种防腐剂含量的分析。
A reversed phase high performance liquid chromatographic （RP-HPLC）method has been developed for the simultaneous determination of twenty-four preservatives in cosmetics. A Kromasil C18 column was used with phosphate buffer solution （ pH = 4.26 ） as the mobile phase under the condition of gradient elution. Preservatives were extracted with methanol by ultrasonic, then analyzed by RP-HPLC-diode array detection. The pre-treatment method of sample and the chromatographic condition of analysis were critically examined. The relative standard deviations（RSD） were 1.3% - 3.3% , recoveries were 90.6% - 97.8% , all the corre- lation coefficients（r） were no less than 0. 9997. The method was precise and accurate, and could be used for analyzing the preservatives in cosmetic products.
出处
《分析化学》
SCIE
EI
CAS
CSCD
北大核心
2007年第10期1439-1443,共5页
Chinese Journal of Analytical Chemistry
关键词
反相高效液相色谱
化妆品
防腐剂
Reversed phase high performance liquid chromatography, cosmetics, preservative
作者简介
E—mail：ppwtpp@126．com
