摘要
探讨人脐血单核细胞在髓系树突状细胞(DC)体外诱导体系中获得的CD123+髓系DC的生物学特性。分离脐血单核细胞,用人重组的粒/单核细胞集落刺激因子(GM-CSF)和IL-4将其诱导为DC。用流式细胞仪检测DC表面共刺激分子、CD123和CD11c的表达,并用间接免疫磁珠法将其中CD123+DC加以分离纯化;激光共聚焦显微镜、扫描电镜和倒置显微镜观察CD123+DC形态;3H-TdR渗入法检测CD123+DC对同种异体T细胞的刺激能力。脐血单核细胞经GM-CSF和IL-4诱导7 d后,细胞表面高度表达HLA-DR、CD86、CD11c和CD123,低表达CD83,丧失CD14的表达,其中CD123和CD11c均匀分布于DC表面。免疫磁珠纯化后的CD123+DC呈现不成熟DC形态,除细胞体积较小外,其表面突起类似于CD123?DC。CD123+DC能明显刺激同种异体T细胞增殖,但其刺激能力较CD123?DC组低(P<0.05)。GM-CSF和IL-4培养体系中的CD123+DC可能是DC分化发育过程中更早期的未成熟髓系DC,具有独特的生物学特性。
To investigate the biological properties of CD123^＋ DC derived from cord blood monocytes in myeloid culture system, the monocytes isolated from cord blood were cultured with GM-CSF and IL-4 for 7 days to induce DC, and the related phenotype of DC was analyzed by flow cytometery. CD123^＋ DC were purified with magnetic cell sorting separation technique. The morphological characteristics of CD123^＋ DC was observed under confocal laser-scanning microscope and scan electron microscope. Immuhe function was evaluated by allogeneic mixed T lymphocyte reaction by 3 H-TdR assay, in which the ratio of DC and T cells were 1： 10. FACS analysis showed the high expression of HLA-DR, CD86, CDllc and CD123, low levels of CD83 on DC after induction by GM-CSF and IL-4 for 7 days, while the expression of CD14 was rather low. Well-distributed CD123 and CDllc were detected on DC. Purified CD123^＋ DC showed typical morphology of immature DC with similar short cytoplasmic projections as CD123^- DC. CD123^＋ DC stimulated the proliferation of allogeneic T cells, but the stimulated effect of CDI＋ DC was lower than that of CD123^- DC（P〈0. 05）. It is apparent that the CD1234 DC induced with GM-CSF and IL-4 might be the immature myeloid DC of earlier phase along with the DC differentiation and with special biological properties.
出处
《现代免疫学》
CAS
CSCD
北大核心
2007年第5期382-385,共4页
Current Immunology
基金
上海市青年科技启明星课题(02QB14035)
上海市卫生局青年课题资助项目(024Y14)
作者简介
石军，女，副教授．副主任医师，主要从事树突状细胞与造血干细胞移植的研究。E-mail：shijun7@hotmail．com
