摘要
目的:研究茅苍术挥发油超临界CO2萃取的最佳工艺条件和挥发油的化学成分。方法:以挥发油得率为指标,采用正交试验优选超临界CO2萃取茅苍术挥发油的最佳工艺;气质联用法(GC-MS)分析挥发油化学成分。结果:超临界CO2萃取茅苍术挥发油的最佳工艺为:萃取时间3.5 h,萃取温度45℃,萃取压力30 MPa,流量3 L/m in,挥发油得率为13.80%。GC-MS分析结果显示84个色谱峰,鉴定出67种化学成分,占总馏出峰面积的96.07%。含量最高的为苍术酮(24.92%),其次为苍术素(22.26%),β-桉叶醇(10.67%)等。结论:超临界CO2萃取茅苍术挥发油具有得率高,萃取时间短,温度低,无溶剂残留等优点。
Objective ： To study the optimal technical condition of extracting the essencial oil with supercritical CO2 from Atractylodes lancea （ Thunb. ） DC. and chemical composition in the oil. Methods： Optimized the technology of extracting the essencial oil with supercritical CO2 from Atractylodes lancea （ Thunb. ） DC. by simple factor and orthogonal experiment as guided by the yield of the essencial oil ; GC-MS analyzed chemical composition. Results： The optimum technology of extracting the essencial oil with supercritical CO2 was as follows ： 3.5 h as extraction time, 45 ℃ as extraction temperature, 30 MPa as extraction pressure, 3 L/min as flow rate, 13.80% as yield . The analytic result of GC-MS displayed 84 chromatographic peaks, identified 67 chemical compositions, that was 96.07 percent of whole run-down peak area. The supreme content was atractylone（24.92% ） , next atrctylodine（22.26% ） , β-eudesmol（ 10.67% ）and so on. Conclusion： The SFE-CO2 was of advantage of： high yield, short extraction time,low temperature and without residue of solvent.
出处
《江苏大学学报（医学版）》
CAS
2007年第5期393-395,共3页
Journal of Jiangsu University：Medicine Edition
基金
江苏省自然科学基金资助项目(BK2004062)
关键词
茅苍术
挥发油
正交试验
超临界萃取
Atractylodes lancea（Thunb. ） DC.
essential oil
orthogonal experiment
SFE
作者简介
杨凌（1979-），女，硕士研究生；
欧阳臻（联系人），教授，硕士生导师，E-mail：yshkong@ujs．edu．cn
