摘要
通过使用带正电荷的ZnO溶胶-凝胶在玻碳电极表面固定酶，研制了一种简单有效的酪氨酸酶传感器。结果表明，ZnO溶胶-凝胶的等电点为酪氨酸酶的固定提供了有利的微环境，酪氨酸酶能很好地保持其生物活性。所研制的传感器达到95％稳定状态电流的时间在10S以内。酚类化合物可通过酶催化产生的醌在-200mV（对饱和甘汞电极）直接还原而测定，传感器对苯酚测定的灵敏度为168μA·mmol^-1·L^-1，线性范围为1．5×10^-7～4．0×10^-5mol·L^-1，检出限为8．0×10^-8mol·L^-1。该传感器使用二周后活性仍保持原有活性的75％。
A simple and effective tyrosinase biosensor was developed based on the immobilization of tyrosinase in a positively charged ZnO sol-gel matrix on a glass carbon electrode. It has been found that the ZnO sol-gel matrix provided an advantageous microenvironment in terms of its favorable isoelectric point for tyrosinase loading, in which the immobilized tyrosinase retained its activity to a large extent. Phenolic compounds were determined by the direct reduction of biocatalytically generated quinone species at -200 mV （vs. SCE）. The resulting biosensor could reach 95% of steady-state current within 10 s. The sensitivity for phenol determination was 168μA.mmol^-1.L^-1 , and a linear response were from 1.5 ×10^-7 to 4.0×10^-5 mol. L^-1 with a detection limit of 8.0×10^-8 mol. L^-1. The biosensor response retained 75% of the initial response after two weeks.
出处
《分析科学学报》
CAS
CSCD
2007年第5期555-558,共4页
Journal of Analytical Science
基金
河南工业大学博士基金(150196)
作者简介
通讯联系人：刘志敏，博士，副教授，主要研究方向为电化学．
