摘要
采用MTT法及Comet assay(彗星实验分析方法)研究了表没食子儿茶素没食子酸酯(EGCG)与卡莫司汀联用对人肺癌细胞A549的生长抑制率及DNA损伤的影响,结果表明:联合应用EGCG能降低卡莫司汀对人肺癌细胞A549的IC50。卡莫司汀单用对人肺癌细胞A549的IC50为187.46μg/ml,当用无毒浓度25μg/ml和50μg/ml的EGCG与卡莫司汀联用时,卡莫司汀对人肺癌细胞A549的IC50分别下降为139.56μg/ml和154.02μg/ml。EGCG还能增强卡莫司汀引起的人肺癌细胞A549 DNA的损伤,彗星尾矩值由单用时的19.02±14.60上升为联用时的33.07±10.47。结果说明EGCG能增强卡莫司汀对人肺癌细胞A549造成的DNA损伤,从而增强其抗肿瘤活性。
The effect of （-）-epigallocatechin gallate （EGCG） combined with carmustine on the growth inhibition and DNA damage of human lung carcinoma cell line A549 were evaluated by MTT assay and Comet assay repectively. The results showed that 50% inhibitory concentration （IC50） of carmustine to A549 human lung carcinoma cell line was decreased when combined with EGCG. The 50% inhibitory concentration （IC50） of carmustine alone to human lung carcinoma cell line A549 was 187.46 μg/ml, When the carmustine is combine with EGCG（25 μg/ml and 50μg/ml, the concentration under which EGCG has no growth inhibition to A549）, however, the IC50 value to A549 cell line decreased to 139.56 μg/ml and 154.02 μg/ml respectively. EGCG also enhanced carmustine-induced DNA damage in A549 cell line by Comet assay. The tail moment increased from 19.02±14.60 to 33.07±10.47. So, EGCG can enhance carmustine-induced DNA damage in A549 cell line, hence enhance the anticancer activity of carmustine.
出处
《茶叶科学》
CAS
CSCD
北大核心
2007年第4期302-306,共5页
Journal of Tea Science
基金
电子科技大学杰出人才引进项目JC901002
作者简介
黄秀华（1970-），女，重庆梁平人，博士生，从事抗菌抗肿瘤和基因毒性方面研究。
通讯作者：任正隆renzl@uestc．edu．cn
