摘要
研究东北虎毛发DNA的水煮抽提法,并将传统的酚仿抽提法和水煮法进行比较。水煮法即通过对细胞的煮沸和冷却,使细胞破裂、蛋白质变性,从而获得用于PCR扩增的模板DNA。通过测定OD值并计算浓度和纯度,同时进行琼脂糖电泳检测、PCR扩增和PAGE电泳检测,分析2种方法提取东北虎毛发DNA的质量差异。结果表明:水煮法提取东北虎毛发中DNA是一种快捷、简便、经济、高效的方法。
The DNA extraction from the hairs of the Panthera Tigris Altaica, cooking extraction method in water was studied, and the comparison with the traditional phenol and chloroform method was done. The cooking extraction method was the method from which genomic DNA was obtained when the cells were broken and proteins were denatured after the cells were cooked and cooled quickly. The extracting results of the two methods were detected through agrose gel electrophoresis, PCR amplification and PAGE gel electrophoresis, the OD value, density and purity. The analysis result indicated that the cooking extraction method in water is a quick, simple, economic and efficient method to extract DNA from the hairs of the Panthera Tigris Altaica.
出处
《中国畜牧兽医》
CAS
2007年第9期33-35,共3页
China Animal Husbandry & Veterinary Medicine
基金
江苏省六大人才高峰项目
关键词
东北虎
毛发
DNA
酚仿抽提法
水煮抽提法
Panthera Tigris Altaica
hair
DNA
phenol and chloroform method
cooking extraction method
作者简介
吴云良（1981-），男，江苏人，硕士生，研究方向：动物遗传资源的研究、保护与利用。
通讯作者：陈国宏（1963-），男，江苏人，教授，博士生导师，研究方向：动物遗传资源的研究、保护与利用。Tel：0514—7979230 Email：ghchen@mail．yzu．edu．cn
