摘要
针对普通CCD拍摄的低对比度生物样品图像,设计并实现了一种以PC机为控制核心的光学显微镜自动聚焦系统。系统采用弹性联轴器与显微镜调焦轴直接耦合的方式,结合细分驱动,有效地消除了传动机构的回程差,提高了聚焦控制精度。在对几种常用图像清晰度评价算子进行分析的基础上,证实了图像自相关算子是评价低对比度生物样品图像清晰度的有效算法。实验结果表明,该系统自动聚焦的准确度和实时性基本上满足生物学研究的需要。
A novel optical auto-focus microscopy controlled by PC is presented,especially for the low-contrast images from con- ventional CCD camera.Firstly,by directly matching flexible shaft coupling and focusing axis of microscope,integrating subdivision mechanism of micro-step driver,the mechanical tolerance of gear was diminished while the accuracy of focus was improved effectively.Secondly,it has been proved that the auto-correlation operator is one effective approach to evaluate low-contrast image specially based on the performance comparison of some image analysis operators.Biology experiments have shown that the pro- posed scheme meets the requirement of auto-focusing both on accuracy and speed.
出处
《计算机工程与应用》
CSCD
北大核心
2007年第27期119-120,215,共3页
Computer Engineering and Applications
基金
国家自然科学基金(the National Natural Science Foundation of China under Grant No.60410131
No.90508003)
关键词
自动聚焦
显微镜
步进电机
自相关算子
auto-focus
microscope
stepper-motor
auto-correlation operator
作者简介
易秋实（1982-）,男,硕士研究生,主要研究方向：显微成像系统设计;E-mail：ibp@mail.hust.edu.cn
曾绍群（1969-）,通讯作者,男,博士,教授,博士生导师.
