摘要
目的探讨雌激素对去卵巢(OVX)大鼠骨丢失的作用敏感部位。方法8月龄SD大鼠去卵巢后,皮下注射17β-雌二醇(E2)(10、30μg.kg-1.d-1)3月,双能X线骨密度仪检测全身总骨密度(BMD),离体腰椎(L4-6)、股骨、胫骨BMD。实验分为假手术组、去卵巢模型组、雌激素小剂量组、雌激素大剂量组,每组10只。结果E2可逆转OVX所致总BMD降低,增加OVX后腰椎整体及3个兴趣区BMD。E2治疗可升高OVX后股骨BMD,其中股骨整体、股骨近端和股骨远端处改变最为明显(P<0.01),其余各兴趣区BMD改变相对稍弱,而股骨中段最不敏感。胫骨BMD指标中以胫骨近端最为敏感(P<0.01),而中远端几乎无改变。结论雌激素对去卵巢(OVX)大鼠的作用敏感部位与骨量丢失敏感部位具有一致性,雌激素增加骨密度主要在腰椎、股骨近端、股骨远端和胫骨近端。
Objective To investigate the sensitive regions of the effect of estrogen on the bone mineral density （BMD） in ovariectomized rats. Methods 17β-estradiol （E2） had been administered （10,30 μg·kg^-1 ·d^-1, s. c. ） in ovariectomized 8-month old SD rats for 3 months. Total body BMD and BMD in isolated lumbar part of spines （L4-6）, the femurs and the tibias post-treatment were measured by dual-energy X-ray absorptiometry. Forty rats were randomly divided into four groups including sham, OVX （ovariectomy）, E2 （ 10μg·kg^-1 · d^-1 ）, E2 （30 μg· kg^-1· d^-1 ） group. Results The administration of E2 may reverse the decreased BMD of total body and L4-6 after ovariectomy. BMD in total femurs, distal and proximal femurs metaphysis are most apparently increased after E2 treatment in OVX rats, and BMD in other femur interest regions are weakly changed, especially in middle part of femurs. In tibias, the proximal metaphysis are most sensitive to E2 treatment, and there were no significant changes in middle to distal part of tibias. Conclusions The sensitive regions of the effect of estrogen are in accordance with bone lost after ovariectomy in rats. The increase of BMD after treatment by E2 is mainly present in lumber part of spines, the proximal and distal metaphysic in femurs, and the proximal metaphysic in tibias.
出处
《中国骨质疏松杂志》
CAS
CSCD
2007年第2期108-111,共4页
Chinese Journal of Osteoporosis
基金
国家自然科学基金项目(30500184)
关键词
雌激素
去卵巢大鼠
骨密度
敏感部位
腰椎
股骨
胫骨
Estrogen
Ovarictomized rats
Bone mineral density（BMD）
Sensitive regions
Lumber
Femur
Tibia
作者简介
彭维杰,通讯作者;Email：weijiepeng@126.com
