摘要
目的探讨ｐ１６基因缺失和突变与人非小细胞肺癌发生、发展的关系。方法应用多重ＰＣＲ、ＳＳＣＰ分析及ＤＮＡ序列分析技术，对４５例人非小细胞肺癌组织中ｐ１６基因外显子１和外显子２进行基因缺失和突变分析。结果４５例肺癌样品中共检出１９例ｐ１６基因缺失，总的缺失率为４２．２％（１９／４５）。其中纯合型缺失８例（１７．８％），半合型缺失１１例（２４．４％）；外显子１缺失７例（１５．６％），外显子２缺失１２例（２６．７％）；ｐ１６基因外显子２的突变检出率为３１．１％（１４／４５）；ＤＮＡ序列分析显示ｐ１６基因７２位密码子无义突变７例，７５位密码子错义突变７例。结论ｐ１６基因的缺失和突变可能与人非小细胞肺癌的发生、发展有关。
Objective To detect the relationship between p16 gene deletion and mutation and oncogenesis and development of human non small cell lung cancer. Methods Deletion and mutation at p16 gene exons 1 2 were determined in 45 human non small cell lung cancer samples with multiple PCR, SSCP and DNA sequence. Results The results showed that 19 cases had p16 gene deletion and that the deletion rate was 42.2%(19/45). Among whom, 8 cases had homozygous deletion (17.8%) and the other 11 cases had partial deletion(24.1%); 7 cases had deletion in exon 1 and the other 12 cases had deletion in exon 2. The SSCP analysis showed that the mutation rate at p16 exon 2 was 31.1%(14/45). DNA sequencing revealed that nonsense mutation at p16 codon 72 was found in 7 cases and missense mutation at p16 75 codon was found in 7 cases in this series. Conclusion Deletion and mutation of p16 gene may be related to the oncogenesis and development of human non small cell lung cancer.
出处
《中国胸心血管外科临床杂志》
CAS
1997年第2期67-69,共3页
Chinese Journal of Clinical Thoracic and Cardiovascular Surgery
关键词
非小细胞肺癌
P16基因
突变
肺肿瘤
Non small cell lung cancer p16 gene Mutation Gene deletion
