摘要
目的:在体外初步探讨联合用药可缩小单药对细菌的耐药突变选择窗(MSW),为临床合理使用现有抗菌药物,防止细菌耐药产生提供理论依据。方法:应用肉汤法富集1010CFU/mL细菌,琼脂平板二倍稀释法测定左氧氟沙星、万古霉素单药和两药联用对金黄色葡萄球菌ATCC29213的最低药物浓度(MPC)和MSW。结果:左氧氟沙星、万古霉素单药对金黄色葡萄球菌ATCC29213的MSW分别为16和64。万古霉素和左氧氟沙星联合用药(1MIC+8MIC,2MIC+4MIC)使左氧氟沙星单药对ATCC29213的MSW缩小2～4倍。左氧氟沙星联合万古霉素用药(1MIC+16MIC,2MIC+8MIC)使万古霉素对ATCC29213的MSW缩小4～8倍。结论:万古霉素和左氧氟沙星联合用药可缩小各自单药对金黄色葡萄球菌ATCC29213的MSW。
AIM： To study whether levofloxacin and Vancomycin combination can narrow mutant selection window（MSW） of single drug group in vitro, which contribute to new strategies for restricting the development of resistance and provide data for rational applications of available antibacterial drugs in clinic practice. METHODS： Minimal antibiotic concentration（MPC） and MSWs were determined by spreading 10^10 CFU/mL coccus on agar plates at different drug concentrations. MPC was defined as the lowest concentration inhibiting 100% of the coccus population after 72 h incubation. RESULTS： MSW of levofloxacin and vancomycin for staphylococcus aureus strain ATCC29213 was 16 and 64 respectively; Vancomy-cin combined with levofloxacin（ 1MIC ＋ 8MIC, 2MIC ＋ 4MIC） decreased the MSW of levofloxacin to staphylococcus aureus ATCC29213 for 2-4 times; Levofloxacin combined with vancomycin （1MIC ＋ 16MIC, 2MIC ＋ 8MIC） decreased the MSW of vancomycin to staphylococcus aureus ATCC29213 for 4 - 8 times. CONCLUSION： Combinations of levofloxacin and vancomycin can decrease MSW to staphylococcus aureus, ATCC29213 compared with individual drug administration.
出处
《中国临床药理学与治疗学》
CAS
CSCD
2007年第8期911-914,共4页
Chinese Journal of Clinical Pharmacology and Therapeutics
作者简介
李朝霞,女，博士研究生，主治医师，研究方向：肺部感染性疾病和细菌耐药机制。Tel：010-66937090 E-mail：lzx99@vip．sina.com
刘又宁．通讯作者，男，医学博士，教授，博士生导师，研究方向：肺部感染性疾病和呼吸衰竭的诊断治疗。Tel：010-66939366 E-mail：liuyn@301hospital．com．cn
