摘要
目的探讨环境雌激素多氯联苯同系物PCB153对发育过程中小鼠胚胎细胞凋亡的影响。方法于体外施予孕8.5d小鼠胚胎不同浓度PCB153,应用植入后全胚胎培养技术培养胚胎;采用原位TUNEL技术观察胚胎细胞凋亡。结果PCB153对胚胎发育具有明显影响且呈剂量-效应关系,且随PCB153浓度增加,凋亡细胞比值上升。结论PCB153能诱导胚胎细胞凋亡,这与其致胚胎发育异常有关,可能是PCB153的致畸作用机制之一。
Objective To explore the effects of polychlorinated biphenyl congener （ PCB153 ） an environmental estrogen, on embryonic development and its mechanism. Methods Mouse embryos of gestational day 8.5 were exposed to different doses of PCB153 ex vitro. Post-implantation whole embryo culture （WEC） technique was used to investigate the effects of PCB153 on growth and development. The terminal deoxynucleotidyl transferase （TdT）-mediated dUTP nick-end labelling （TUNEL） technique was used to detect apoptosis in embryonic tissue sections. Results PCB153 had obvious effects on the development and growth of embryos in a dose-dependent manner; The ratio of apoptotic cells was increased with increased doses of PCB153. Conclusion PCB153-induced apoptosis is involved in mouse embryo anomalies.
出处
《第三军医大学学报》
CAS
CSCD
北大核心
2007年第19期1838-1840,共3页
Journal of Third Military Medical University
基金
重庆市科技计划项目(2002-10-77)~~
关键词
植入后全胚胎培养
PCB153
胚胎发育
细胞凋亡
致畸机制
post-implantation whole embryo culture
PCB153
embryonic development
apoptosis
teratogenicmechanism
作者简介
常青（1963-），女，湖南省长沙市人，博士，主任医师，教授，主要从事出生缺陷方面的研究，发表论文16篇。电话：（023）68765399．E-mail：cqli99@yahoo．com．cn
