摘要
采用液体双相分层法和等电点沉淀法提取苏云金芽孢杆菌伴胞晶体蛋白．原子力显微镜观测，发现液体双相分层提取的晶体蛋白具有完整典型的晶体结构；等电点法提取的得率高，不能检测到完整的晶体结构．聚丙烯酰胺凝胶电泳分析，等电点法提取的蛋白质含量是液体双相法提取的蛋白含量的1～2倍．将2种方法提取的晶体蛋白对初孵棉铃虫幼虫生物测定均表现杀虫生物活性，经统计分析，液体双相法提取的蛋白质生测毒力比等电点法的高1．5～3倍．
It is reported that a comparison with the different of extracting parasporal Crystal Protein of Bacillus thuringiensis between the. liquid double-phase method and Isoelectric point method. Using atomic force scan （AFM） electron microscope as stool, It is found that proteins extracted by liquid double-phase method have the typical shape and structure,while that of extracted by isoelectric point method have no typical crystal shape. However, when analyzed by SDS-PAGE, the result show that pure protein content extracted by the Liquid double-phase method is 1 - 2 times less than that of extracted by isoelectric point method. The bioassay is performed on proteins extracted by two different methods. The result shows that proteins extracted by either of the two methods work effectively on young instar larvae of Helicouerpa armigera. But statistic analysis show that proteins extracted by liquid double-phase was 1.5 - 3 times more toxic than that of extracted by isoelectric point.
出处
《湖南师范大学自然科学学报》
CAS
北大核心
2007年第3期94-98,共5页
Journal of Natural Science of Hunan Normal University
基金
国家自然科学基金资助项目(30670052)
国家863计划资助项目(2006AA02Z187
2006AA10A212)
湖南省自然科学基金重点资助项目(06jj2009)
作者简介
罗朝辉（1981-），男，湖南衡南县人，湖南师范大学硕士研究生．
通讯作者：dxz963@tom．com
