摘要
建立一种以固相萃取（SPE）、高效液相色谱（HPLC）为基础的测定梨果皮、果肉中吡虫啉残留量的方法。样品用乙腈进行提取,经二氯甲烷液液分配后用阳离子交换固相萃取（SCX-SPE）柱净化,高效液相色谱/二极管阵列检测法（HPLC/DAD）测定。各种添加水平（果皮：0.05、0.1、0.5、2 mg/kg;果肉：0.05、0.5、1mg/kg）的回收率为83%~103%,相对标准偏差（RSD）小于10%,方法的检出限为0.05 mg/kg。运用此方法分别对梨果皮和果肉中吡虫啉的残留动态进行研究,发现吡虫啉主要在梨果皮中残留,得到吡虫啉在果皮中的消解动态方程为：wt=w0×exp（-0.235t）,半衰期是2.98 d。
A method was developed for the determination of imidacloprid residue in the pericarp and pulp of pear with SCX-solid phase extraction（SPE）-high performance liquid chromatography（HPLC）.The sample was extracted with acetonitrile and the extract was clean-up with SCX-SPE after LLE with dichloromethane;the content of imidacloprid was determined by HPLC with diode-array detection.The average recoveries of imidacloprid in pear pericarp（spiked at 0.1,0.5,and 1 mg/kg） and pear pulp（spiked at 0.05,0.5 and 1 mg/kg） ranged from 83% to 103% with relative standard deviation（RSD〈10%）.The detection limit was 0.05 mg/kg.The results of the study of the degradation dynamic of imidacloprid residue indicated that the residue was mostly left in the pear pulp.
出处
《分析测试学报》
CAS
CSCD
北大核心
2007年第5期682-685,共4页
Journal of Instrumental Analysis
作者简介
吴迪（1983-），女，吉林临江人，硕士研究生；李重九，联系人．Tel：010—62733079，E—mail：wudii098@163．com
