摘要
BMP2/7异源二聚体的活性显著高于BMP2同源二聚体,但其机制并不清楚。采用哺乳动物细胞表达的BMP2/7异源二聚体处理成骨细胞MC3T3-E1,细胞化学染色发现BMP2/7的活性显著高于BMP2,报告载体p3GC2-LUX检测发现BMP2/7能够明显上调BMP/Smad通路的活性(P<0.05)。但在成骨细胞中过表达CIZ(casinteracting zinc finger protein),能够显著抑制BMP2/7上调ALP与Osteocalcin的作用,并阻断BMP2/7对BMP/Smad通路的激活。同时发现BMP蛋白能够上调CIZ的表达,但BMP2/7的作用明显低于BMP2同源二聚体。可以认为BMP2/7能够诱导CIZ的表达,但由于作用较弱,所以对自身活性的反馈抑制作用也较弱,这可能是BMP2/7有着较强生物活性的关键所在。
BMP2/7 heterodimer has stronger bioactivity than BMP2 homodimer, but the mechanism remains to be unclear. Treating the osteoblast MC3T3-E1 cells with BMP proteins, results of Alkalin phosphatase staining, Alizarin red staining, as well as p3GC2-LUX luciferase assay demonstrated that BMP2/7 heterodimers significantly promoted osteogenic differentiation to a higher extent than BMP2 homodimers. Overexpression of CIZ in MC3T3-E1 significantly inhibited the promotion of ALP and Osteocalcin expression by BMP2/7, and blocked activation of BMP/Smad. BMP protein may induce the expression of CIZ, but effects of BMP2/7 heterodimer is weaker than BMP2 homodimer. It suggests that the effects of BMP2/7 on CIZ expression is related to its bioactivity.
出处
《中国生物工程杂志》
CAS
CSCD
北大核心
2007年第9期14-18,共5页
China Biotechnology
基金
广州市科技计划资助项目(2006Z1-E0031
2006Z3-E0791)
作者简介
通讯作者，电子信箱：sunfenyong@263．net
