摘要
目的:探讨大蒜素对体外培养结肠癌HT29细胞增殖的影响,并探讨其作用机制。方法:采用四甲基偶氮唑蓝(MTT)法测定不同浓度大蒜素对HT29细胞增殖抑制率;流式细胞术测定细胞周期变化及凋亡相关蛋白Bcl-2与Bax的表达。结果:MTT显示大蒜素能抑制人结肠癌HT29细胞的增殖,并具有时间-剂量依赖性;流式细胞术测定结果显示10μg/ml大蒜素可明显诱导HT29细胞凋亡,并将细胞周期阻滞在G2/M期;可降低癌基因蛋白Bcl表达,而促进Bax表达。结论:大蒜素对人结肠癌HT29细胞增殖具有抑制作用,可能与细胞周期阻滞和凋亡相关蛋白表达有关。
Objective： To study the affection of Diallyl trisulfide on cultured human colon carcinoma cell line HT-29 and its mechanism, Methods： The viability of cells after various treatments were determined using the method of MTT. Flow cytometry were used to analyze the change of cell cycle and Bcl-2, Bax levels.Resusts： The proliferation of human colon carcinoma HT-29 cells were inhibited by Diallyl trisulfide. The inhibition was associated with dose and time dependence. Flow cy tometry results showed that： 1） When cells were treated with Diallyl trisulfide at the concentration of 10 μg/mL for 12 h and 24 h, the percentage of G0/G1 phase cells was decreased and that of G2/M phase cells was significantly increased compared with those in the control group.2） Diallyl trisulfide down regulated Bcl expression, while up-regulated bax expression. Conclusion It was suggested that Diallyl trisulfide inhibited HT29 cell proliferation. And these were associated with arrest of cell cycles and down regulation of Bcl2/Bax ratio.
出处
《中国现代普通外科进展》
CAS
2007年第4期306-309,共4页
Chinese Journal of Current Advances in General Surgery
关键词
大蒜素
结肠癌细胞系T29
凋亡
流式细胞术
Diallyl trisulfide
Human colon carcinoma cell line HT-29
Apoptosis
Flow cytometry
作者简介
王旭平（1979-），女，硕士，技师。Tel：13589033572 E-mail：wangxuping@126．com
[通讯作者]辛华，女，教授，山东大学医学院细胞生物学研究所。
