摘要
目的建立HPLC法测定复方氨酚苯海拉明片中盐酸麻黄碱和盐酸苯海拉明的含量。方法用Kromasil C18（250mm×4．6mm，5μm）色谱柱；流动相甲醇-乙腈-1％十二烷基硫酸钠溶液（26：32：42）（冰醋酸调pH值3．1），检测波长257nm。结果线性范围，盐酸麻黄碱：1．2288～11．0592μg，r=0．9999，盐酸苯海拉明：1．2168～10．9512μg，r=1．0000；平均回收率，盐酸麻黄碱98．79％，RSD％：0．59％，盐酸苯海拉明100．44％，RSD％=0．57％。结论本方法简便、准确、灵敏度高、重现性好，可更好地控制本品的质量。
Objective To establish a method for the determination of ephedrine hydrochloride and diphenhydramine hydrochloride in compound Anfenbenbenhailaming tablets by high performance liquid chromatography （ HPLC ）. Methods Kromasil C18 （250 mm×4.6 mm, 5 μm） column was used and the mohile phase was methanol-acetonitrile -1% sodium laurylsulfate solutiorl （26： 32：42 ） （ pH was adjusted to 4.5 with acetic acid glacial）. The detection wavelength was 257 nm. Results The linear range was as follows ： that of ephedrine hydroehloride was 1. 228 8 - 11. 059 2 μg （ r = 0. 999 9 ） , and that of diphcnhydramine hydrochloride was 1. 216 8 - 10.951 2 μg （ r = 1. 000 0）. The recovery rate of ephedrine hydrochloride was 98.79% （ RSD% = 0.59% ） , and that diphenhydramine hydrochloride was 100.44% （ RSD% = 0.57% ）. Conclusion The method of HPLC is simple, accurate, highly sensitive and reproducible, and can provide a reference standard for the quality control of Ankabuangmin tablets or capsules.
出处
《临床军医杂志》
CAS
2007年第5期754-755,共2页
Clinical Journal of Medical Officers
关键词
复方氨酚苯海拉明
盐酸麻黄碱
盐酸苯海拉明
含量测定
compound Anfenbenbenhailaming tablet
ephedrine hydrochloride
diphenhydramine hydrochloride
content determi-nation
作者简介
修培（1966-），女，沈阳人，主管药师。
