摘要
以16个水稻品种为材料,测定了铜对水稻种子萌发相关指标,并观察了根尖有丝分裂及分生区Ca分布的影响。结果表明:(1)0.1 mM和0.2 mM CuSO4溶液处理对水稻根长的抑制作用大于对芽长的抑制,且对不同品种根长的抑制作用存在明显差异;(2)随着铜处理浓度(25、50、75、100μM)的升高,根尖细胞有丝分裂指数及相对有丝分裂指数皆呈下降趋势;(3)正常生长条件下,水稻根尖分生区细胞中的Ca主要分布在液泡和细胞间隙中,而细胞质、细胞核中分布很少;50μM铜处理下,根尖分生区细胞液泡和胞间的Ca沉淀颗粒明显减少,而胞质及核基质中的Ca沉淀颗粒增多。铜胁迫造成根尖细胞中原有Ca分布的变化可能是引起细胞功能的紊乱,进而影响根系生长的原因之一。
The indexes related to seed germination and observing the cell mitosis and Ca^2＋ localization in cells of rice root tips were measured with 16 commonly cultured rice cultivars in this study. The results showed： （1） the root elongation was more easily inhibited than coleoptile one by copper stress （0.1 mM and 0.2 mM）, and the effect of copper on root elongation differed markedly among cuhivars. （2） both the mitotic index and the relative mitotic index decreased with the increase of copper concentration （25,50,75,100μM）. （3） When rice seedlings grew under the normal condition, it was shown that the deposits of calcium antimonite, being indicator for Ca^2＋ localization, were mainly concentrated to the vacuoles and intercellular spaces, and the free Ca^2＋ in cytoplasm and nucleus was very low under the normal condition. When the seedlings were treated with the 50μM CuSO4 ,the Ca^2＋ level of cytoplasm and nucleus in cells of rice root tips increased, while the level in vacuole and intercellular spaces decreased. Based on the above observations, the author proposed that the changes of Ca^2＋ localization in the cells of rice root tips could disturb cellular metabolism by disrupting Ca^2＋ homeostasis and affect the growth of roots.
出处
《科技通报》
2007年第5期664-669,688,共7页
Bulletin of Science and Technology
基金
"十一五"国家科技支撑计划项目(2006BAK02A18)
国家重点基础研究发展规划(973)项目(2002CB10804)
关键词
铜
水稻
萌发
根尖
有丝分裂
Ca分布
copper （Cu）
rice （Oryza sativa L.）
root tip
mitotic index
localization of calcium
作者简介
孙仲秧（1981-），女，浙江宁波人，硕士研究生，主要从事植物环境生理研究。
通讯作者：E-mail：pzhch@zju．edu、cn．
