摘要
利用体外消化法进行尿素与乙酰氧肟酸(AHA)合理配比筛选.试验分为5组,每组基础饲粮相同,尿素(%)与AHA(mg/kg)添加量分别为:A(1.0与25)、B(1.5与25)、C(2.0与25)、D(1.5与15)、E(1.5与35),每组3个平行,采集安装有瘤胃瘘管羊瘤胃液,分6个时间段(0、1、2、4、6、10h)培养,测定发酵终产物的pH值、氨态氮浓度、干物质消失率.结果表明:1)培养1 h后B、C、D处理组氨氮出现高峰,随后缓慢下降,4 h后急剧下降;A、E处理组在2 h后出现高峰随后缓慢下降,4 h后又急剧下降,E组下降异常迅速.这是由于B、C、D处理组尿素量高,A组尿素低,E组AHA高所造成.2)各处理组不同时间段的pH均在6.01~6.55之间变动,且在培养1~4 h内各组pH值都较高,4 h后急剧下降,发酵产酸在培养管累积和氨氮浓度降低是主要原因.3)B处理组干物质消失率变化比较平缓,且在培养后期消失率呈快速上升趋势.尿素与乙酰氧肟酸的合理配比为1.5%与25 mg/kg.
Five proportions of urea and AHA were designed to select rational diet in vitro. The results indicated that: 1)The pH of all groups ranged from 6.01 to 6.55 and increased from 1-4 h after culture, but declined suddenly at 4 h;2)The peaks of NH3-N concentration of B,C,D group appeared at 1 h,and slowly decreased at other hours;the peaks of A and E group occurred after 2 h,but decreased after 4 h,the concentration of NH3-N in group E decreased more rapidly. 3) The DM disappearance rate of t3 group changed smoothly at the begining,but increased rapidly after 6 h. The results indicated that the reasonable proportion of urea and AHA was 1.5 % and 25 mg/kg.
出处
《甘肃农业大学学报》
CAS
CSCD
2007年第4期16-19,共4页
Journal of Gansu Agricultural University
基金
肉羊成型饲料加工技术研究与产业开发(01cx- 05)
反刍动物系列饲料产品的研制与开发(0030707).
关键词
体外法
氨氮浓度
干物质消失率
脲酶
in vitro
NH3-N concentration
dry matter disappearance rate
urease
作者简介
王虎成(1978-),男,兰州大学在读博士;研究方向为动物营养.
通讯作者:李发弟(1963-),男,甘肃民勤人,教授,博士生导师.E—mail;Lifd@gsau.edu.cn