摘要
研究了芦苇化感物质2-甲基乙酰乙酸乙酯(ethyl-2-methylacetoacetate,EMA)对铜绿微囊藻PCC7806的生长抑制特性以及对微囊藻毒素MC-LR产生和释放的影响.结果表明,EMA在培养1周内对铜绿微囊藻PCC7806具有较强的抑制作用,EC50,7d值为2.0 mg.L-1,但EMA的抑制效果随时间的延长而减弱.整个培养期间,EMA对MC-LR的胞外释放无显著影响.培养7 d后,单位藻细胞内MC-LR的含量随EMA浓度的增加而升高,EMA投加浓度为1.5 mg.L-1时,单位藻细胞MC-LR的含量为25ng.(106个)-1,比对照组增加了39%.但单位体积培养液中MC-LR总量(胞内和胞外的总和)随EMA浓度增加先略微升高后显著降低,EMA投加浓度为3 mg.L-1时,培养液中MC-LR胞内胞外总量为28μg.L-1,约为对照组的一半;16 d后,EMA对单个细胞内MC-LR的含量以及MC-LR总量均无显著影响.
The growth inhibition of ethyl2-methylacetoacetate（EMA） isolated from common reed（Phragmites australis Trin.or Phragmites communis Trin.） on the growth of Microcystis aeruginosa PCC7806 was investigated and the intracellular and extracellular concentration of cyanotoxin（MC-LR） after treatment of EMA were tested.The experimental results indicated that EMA has significant inhibitory effect on the growth of M.aeruginosa PCC7806,and the value of EC50,7d was 2.0mg·L^-1. However,the inhibition declined with the cultivation time.During the whole cultivation period,EMA showed no significant effect on the release of MC-LR from cells to the culture.After 7 days,the amount of intracellular MC-LR per cell unit increased with the increasing of EMA concentration.The amount of MC-LR per cell unit was 25ng·（10^6 cells）^1 after the treatment with 1.5mg·L^-1 EMA,which was increased by 39% compared with the control.The total MC-LR production（including intracellular and extracellular MC-LR） first slightly in
creased and then decreased significantly with the increase of EMA concentration.After the treatment with 3mg·L^-1 EMA,the total MC-LR production was 28μg·L^-1（only half of that in the control）.After 16 days,EMA showed no significant effect on both the amount of MC-LR per cell and the total MC-LR production.
出处
《环境科学》
EI
CAS
CSCD
北大核心
2007年第9期2058-2062,共5页
Environmental Science
基金
NSFC-JST国际重大合作项目
关键词
铜绿微囊藻
蓝藻毒素
MC-LR
2-甲基乙酰乙酸乙酯
水华控制
芦苇
Microcystis aeruginosa
cyanotoxins
MC-LR
ethyl-2-methylacetoacetate
water bloom control
reed（Phragmites australis Trin.or Phragmites communis Trin.）
作者简介
门玉洁（1983～），女，硕士研究生，主要研究方向为富营养化藻类的控制，E-mail：menyj@mails．tsinghua．edu．cn
通讯联系人，E-mail：hyhu@tsingua．edu．cn
