摘要
目的:研究加热诱导成骨肉瘤细胞株(OS-9901)凋亡,为中低温度热疗辅助成骨肉瘤综合治疗提供理论依据.方法:成骨肉瘤OS-9901细胞株分别经不同温度的热疗作用1h,用TUNEL法染色及计数、透射电子显微镜观察其对骨肉瘤细胞株的诱导凋亡作用.同时通过免疫组织化学染色,观察43℃1h热疗对成骨肉瘤细胞株OS-9901的Bcl-2蛋白表达的影响.结果:43℃作用1h的成骨肉瘤细胞株继续培养6h后出现大量异常细胞,透射电镜观察可见有典型的细胞凋亡特征性形态改变.TUNEL法染色后通过计数可见有42%的细胞凋亡比例.免疫组织化学显示,43℃作用1h的成骨肉瘤细胞株6h后Bcl-2蛋白的表达明显减少.结论:43℃作用1h可以诱导成骨肉瘤细胞株OS-9901凋亡.加热后成骨肉瘤细胞Bcl-2表达减少,从而进一步诱导发生细胞凋亡,为热疗诱导肿瘤细胞凋亡的机制提供了部分理论依据.
AIM： To observe the apoptosis of the osteosarcoma cells （ OS-9901 ） induced by hyperthermia and to explore the possible mechanism for mild temperature treatment of osteosarcoma. METHODS： The osteosarcoma 0S-9901 cells were treated with different temperatures for 1 h. The cell apoptosis was analyzed with electron microscope, TUNEL staining and counting. The Bcl- 2 protein expression in the OS-9901 cells was also observed before and after hyperthermia （43℃, 1 h） using the immunohistochemical technology. RESULTS： A great number of abnormal osteosarcoma cells were observed at 6 h after 43℃ treatment for 1 h. Transmission electron microscopy demonstrated typical apoptosis morphological features of the cells, including size reduction, chromatin condensation, and apoptotic bodies appearance. The highest apoptosis rate reached 42% analyzed by TUNEL staining. The immunohistochemistry showed that the expression of Bcl-2 decreased after treatment with 43℃ for 1 h. CONCLUSION： 43℃ treatment for 1 h induces apoptosis of osteosarcoma cells. Hyperthermia decreases the Bcl-2 protein expression in the osteosarcoma cells, so as to induce the apoptosis of the cells . This study provides some theoretical basis for the mechanism underlying the apoptosis of tumor cells induces by thermotherapy.
出处
《第四军医大学学报》
北大核心
2007年第16期1482-1484,共3页
Journal of the Fourth Military Medical University
基金
国家自然科学基金(30471988)
关键词
高温
诱发
骨肉瘤
细胞凋亡
超微结构
免疫组织化学
hyperthermia, induced
osteosarcoma
apoptosis
ultrastructure
immunohistochemistry
作者简介
单乐群,博士生（导师范清宇），医师．Tel：（029）84777733 Email：drshanlq@fmmu．edu．cn
通讯作者：范清宇，Tel：（029）84777639 Email：bonetm@fmmu．edu．cn
